Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

Daniel Wan; Xiaowei Wang; Ryosuke Nakamura; Marcos J.C. Alcocer; Franco H. Falcone

doi:10.1007/978-1-0716-0696-4_11

Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

Daniel Wan
, Xiaowei Wang
, Ryosuke Nakamura
, Marcos J.C. Alcocer
, Franco H. Falcone

University of Nottingham
Japan Agency for Medical Research and Development
Justus Liebig University Giessen

研究成果: 書籍の章/レポート/Proceedings › 章 › 査読

4 被引用数 (Scopus)

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Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

本文言語	英語
ホスト出版物のタイトル	Methods in Molecular Biology
出版社	Humana Press Inc.
ページ	145-153
ページ数	9
DOI	https://doi.org/10.1007/978-1-0716-0696-4_11
出版ステータス	出版済み - 2020
外部発表	はい

出版物シリーズ

名前	Methods in Molecular Biology
巻	2163
ISSN（印刷版）	1064-3745
ISSN（電子版）	1940-6029

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引用スタイル

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title = "Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum",

abstract = "Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.",

keywords = "Allergy, DsRed, IgE, Luciferase, NFAT-DsRed, RBL, Reporter system, RS-ATL8",

author = "Daniel Wan and Xiaowei Wang and Ryosuke Nakamura and Alcocer, \{Marcos J.C.\} and Falcone, \{Franco H.\}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",

year = "2020",

doi = "10.1007/978-1-0716-0696-4\_11",

language = "英語",

series = "Methods in Molecular Biology",

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AU - Wan, Daniel

AU - Wang, Xiaowei

AU - Nakamura, Ryosuke

AU - Alcocer, Marcos J.C.

AU - Falcone, Franco H.

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.

PY - 2020

Y1 - 2020

N2 - Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

AB - Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

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KW - DsRed

KW - IgE

KW - Luciferase

KW - NFAT-DsRed

KW - RBL

KW - Reporter system

KW - RS-ATL8

UR - https://www.scopus.com/pages/publications/85089301685

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DO - 10.1007/978-1-0716-0696-4_11

M3 - 章

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AN - SCOPUS:85089301685

T3 - Methods in Molecular Biology

SP - 145

EP - 153

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

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