TY - JOUR
T1 - Rapid assay for detecting gyrA and parC mutations associated with fluoroquinolone resistance in Enterobacteriaceae
AU - Nakano, Ryuichi
AU - Okamoto, Ryoichi
AU - Nakano, Akiyo
AU - Nagano, Noriyuki
AU - Abe, Michiko
AU - Tansho-Nagakawa, Shigeru
AU - Ubagai, Tsuneyuki
AU - Kikuchi-Ueda, Takane
AU - Koshio, Osamu
AU - Kikuchi, Hirotoshi
AU - Ono, Yasuo
PY - 2013/9
Y1 - 2013/9
N2 - We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
AB - We developed a PCR-RFLP assay to detect mutations in the quinolone-resistance determining regions of gyrA and parC associated with fluoroquinolone resistance in Enterobacteriaceae. The assay detected mutations associated with reduced susceptibility to fluoroquinolones and may therefore serve as a specific, rapid, inexpensive, and simple testing alternative.
KW - Enterobacteriaceae
KW - Fluoroquinolones
KW - PCR-restriction fragment length polymorphism
KW - Quinolone resistance-determining regions
UR - http://www.scopus.com/inward/record.url?scp=84880929401&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2013.06.019
DO - 10.1016/j.mimet.2013.06.019
M3 - 記事
C2 - 23816531
AN - SCOPUS:84880929401
SN - 0167-7012
VL - 94
SP - 213
EP - 216
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 3
ER -