Cytokine adsorptive property of various adsorbents in immunoadsorption columns and a newly developed adsorbent: An in vitro study

Shigeto Oda, Hiroyuki Hirasawa, Hidetoshi Shiga, Kazuya Nakanishi, Ken Ichi Matsuda, Masataka Nakamura, Hiroyuki Ikeda, Masamune Sakai

研究成果: ジャーナルへの寄稿記事査読

28 被引用数 (Scopus)

抄録

Background/Aims: Cytokines play important roles in the pathophysiology of systemic infl ammatory response syndrome (SIRS) and sepsis. Therefore, some effective measures to remove cytokines from the bloodstream could be effective in the treatment of SIRS and sepsis. The aim of this study was to evaluate the cytokine adsorptive property of various adsorbents for the purpose of the development of new selective cytokine adsorption columns. Methods: The cytokine adsorptive property of adsorbent in a CF-X column, which consists of cellulose beads cross-linked with hexamethylene-di-isocyanate, was compared with those of various adsorbents in currently available immunoadsorption columns, such as Immusorba TR®, Immusorba PH®, Selesorb®, and Lixelle®, in vitro batchwise test using patients' plasma. A newly developed adsorbent, MPCF-X, which was modified by coating the surface of the adsorbent in CF-X with 2-methacryloyloxyethyl phosphorylcholine (MPC), was also tested for its cytokine adsorptive property. Results: The adsorbent in CF-X showed a signifi cantly higher adsorption rate for TNF-α, interleukin (IL)-6 and IL-10 compared with other adsorbents (p < 0.05). Adsorbent in Lixelle® showed good affi nity to TNF-α and IL-8. Especially, the adsorbent in CF-X almost completely removed TNF-α, whereas it also had considerable affi nity to normal IgG. MPCF-X showed decreased affinity to IgG with considerable adsorptive properties to cytokines. Conclusion: Selective cytokine adsorption columns could be developed with improvement of currently available adsorbents. Such a new selective cytokine adsorption column could be clinically applied for the treatment of SIRS/sepsis.

本文言語英語
ページ(範囲)530-536
ページ数7
ジャーナルBlood Purification
22
6
DOI
出版ステータス出版済み - 2004
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