TY - JOUR
T1 - A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo
AU - Sakamoto, Masayuki
AU - Inoue, Masatoshi
AU - Takeuchi, Atsuya
AU - Kobari, Shigetaka
AU - Yokoyama, Tatsushi
AU - Horigane, Shin ichiro
AU - Takemoto-Kimura, Sayaka
AU - Abe, Manabu
AU - Sakimura, Kenji
AU - Kano, Masanobu
AU - Kitamura, Kazuo
AU - Fujii, Hajime
AU - Bito, Haruhiko
N1 - Publisher Copyright:
© 2022 The Author(s)
PY - 2022/2/28
Y1 - 2022/2/28
N2 - Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo.
AB - Genetically encoded calcium indicators (GECIs) are widely used to measure calcium transients in neuronal somata and processes, and their use enables the determination of action potential temporal series in a large population of neurons. Here, we generate a transgenic mouse line expressing a highly sensitive green GECI, G-CaMP9a, in a Flp-dependent manner in excitatory and inhibitory neuronal subpopulations downstream of a strong CAG promoter. Combining this reporter mouse with viral or mouse genetic Flp delivery methods produces a robust and stable G-CaMP9a expression in defined neuronal populations without detectable detrimental effects. In vivo two-photon imaging reveals spontaneous and sensory-evoked calcium transients in excitatory and inhibitory ensembles with cellular resolution. Our results show that this reporter line allows long-term, cell-type-specific investigation of neuronal activity with enhanced resolution in defined populations and facilitates dissecting complex dynamics of neural networks in vivo.
KW - dual-color imaging
KW - Flp/Frt recombination
KW - genetically encoded calcium indicator
KW - in vivo calcium imaging
KW - transgenic mouse
KW - two-photon microscopy
UR - http://www.scopus.com/inward/record.url?scp=85125195878&partnerID=8YFLogxK
U2 - 10.1016/j.crmeth.2022.100168
DO - 10.1016/j.crmeth.2022.100168
M3 - 記事
C2 - 35474964
AN - SCOPUS:85125195878
SN - 2667-2375
VL - 2
JO - Cell Reports Methods
JF - Cell Reports Methods
IS - 2
M1 - 100168
ER -