Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

Daniel Wan; Xiaowei Wang; Ryosuke Nakamura; Marcos J.C. Alcocer; Franco H. Falcone

doi:10.1007/978-1-0716-0696-4_11

Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

Daniel Wan
, Xiaowei Wang
, Ryosuke Nakamura
, Marcos J.C. Alcocer
, Franco H. Falcone

University of Nottingham
Japan Agency for Medical Research and Development
Justus Liebig University Giessen

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

4 Scopus citations

Abstract

Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	145-153
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-0696-4_11
State	Published - 2020
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2163
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Allergy
DsRed
IgE
Luciferase
NFAT-DsRed
RBL
Reporter system
RS-ATL8

Access to Document

10.1007/978-1-0716-0696-4_11

Cite this

@inbook{b9e6e5a0ab164675a2e6f80a82ebed03,

title = "Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum",

abstract = "Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.",

keywords = "Allergy, DsRed, IgE, Luciferase, NFAT-DsRed, RBL, Reporter system, RS-ATL8",

author = "Daniel Wan and Xiaowei Wang and Ryosuke Nakamura and Alcocer, \{Marcos J.C.\} and Falcone, \{Franco H.\}",

note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",

year = "2020",

doi = "10.1007/978-1-0716-0696-4\_11",

language = "英語",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "145--153",

booktitle = "Methods in Molecular Biology",

}

TY - CHAP

T1 - Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

AU - Wan, Daniel

AU - Wang, Xiaowei

AU - Nakamura, Ryosuke

AU - Alcocer, Marcos J.C.

AU - Falcone, Franco H.

N1 - Publisher Copyright: © Springer Science+Business Media, LLC, part of Springer Nature 2020.

PY - 2020

Y1 - 2020

N2 - Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

AB - Determination of allergen-specific immunoglobulin E (IgE) levels in human blood samples is an important diagnostic technology for the assessment of allergic sensitization. The presence of specific IgE in human serum samples can be measured by sensitizing humanized rat basophil leukemia (RBL) cell lines with diluted serum and measuring cellular activation after challenge with the suspected allergens. This has been traditionally performed by measuring the levels of β-hexosaminidase released upon RBL degranulation. Here, we describe the use of two recently developed humanized RBL reporter cell lines, which offer higher sensitivity and are amenable to high-throughput scale experiments.

KW - Allergy

KW - DsRed

KW - IgE

KW - Luciferase

KW - NFAT-DsRed

KW - RBL

KW - Reporter system

KW - RS-ATL8

UR - https://www.scopus.com/pages/publications/85089301685

U2 - 10.1007/978-1-0716-0696-4_11

DO - 10.1007/978-1-0716-0696-4_11

M3 - 章

C2 - 32766972

AN - SCOPUS:85089301685

T3 - Methods in Molecular Biology

SP - 145

EP - 153

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Use of humanized RBL reporter systems for the detection of allergen-specific IgE sensitization in human serum

Abstract

Publication series

UN SDGs

Keywords

Access to Document

Other files and links

Fingerprint

Cite this