TY - JOUR
T1 - Type II phospholipase A2 is linked to cyclooxygenase-2-mediated delayed prostaglandin D2 generation by cultured mouse mast cells following FcεRI- and cytokine-dependent activation
AU - Ashraf, Musharraf
AU - Murakami, Makoto
AU - Shimbara, Satoko
AU - Amakasu, Yoshihisa
AU - Atsumi, Gen Ichi
AU - Kudo, Ichiro
PY - 1996/12/24
Y1 - 1996/12/24
N2 - Treatment of murine bone marrow-derived mast cells (BMMC) with interleukin (IL)-10 and IL-1β increased the expression of type II secretory phospholipase A2 (sPLA2), but not cytosolic PLA2 (cPLA2), after culture for several hours. Further stimulation with IgE and antigen (IgE/Ag) increased cyclooxygenase (COX)-2 expression dramatically, accompanied by augmented delayed prostaglandin (PG) D2 generation over several hours. BMMC were also found to express type IIC PLA2 (PLA2-IIC), a recently described novel sPLA2 possessing 16 cysteine residues, expression of which changed minimally after BMMC activation. Delayed PGD2 generation was suppressed by ~80% by an antibody raised against recombinant murine type II sPLA2. These results suggest that, of the three PLA2s expressed in BMMC, type II sPLA2 is the critical enzyme that is coupled with COX-2-dependent PGD2 generation elicited by IgE/Ag in the presence of IL-10 + IL-1β.
AB - Treatment of murine bone marrow-derived mast cells (BMMC) with interleukin (IL)-10 and IL-1β increased the expression of type II secretory phospholipase A2 (sPLA2), but not cytosolic PLA2 (cPLA2), after culture for several hours. Further stimulation with IgE and antigen (IgE/Ag) increased cyclooxygenase (COX)-2 expression dramatically, accompanied by augmented delayed prostaglandin (PG) D2 generation over several hours. BMMC were also found to express type IIC PLA2 (PLA2-IIC), a recently described novel sPLA2 possessing 16 cysteine residues, expression of which changed minimally after BMMC activation. Delayed PGD2 generation was suppressed by ~80% by an antibody raised against recombinant murine type II sPLA2. These results suggest that, of the three PLA2s expressed in BMMC, type II sPLA2 is the critical enzyme that is coupled with COX-2-dependent PGD2 generation elicited by IgE/Ag in the presence of IL-10 + IL-1β.
UR - https://www.scopus.com/pages/publications/0030600547
U2 - 10.1006/bbrc.1996.1872
DO - 10.1006/bbrc.1996.1872
M3 - 記事
C2 - 8954964
AN - SCOPUS:0030600547
SN - 0006-291X
VL - 229
SP - 726
EP - 732
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 3
ER -