TY - JOUR
T1 - Tissue gene expression of renin-angiotensin system in human type 2 diabetic nephropathy
AU - Konoshita, Tadashi
AU - Wakahara, Shigeyuki
AU - Mizuno, Shinichi
AU - Motomura, Makoto
AU - Aoyama, Chikako
AU - Makino, Yasukazu
AU - Kawai, Yasuyuki
AU - Kato, Norihiro
AU - Koni, Ichiro
AU - Miyamori, Isamu
AU - Mabuchi, Hiroshi
PY - 2006
Y1 - 2006
N2 - OBJECTIVE - Recent studies have proved that blockade of the renin-angiotensin system (RAS) retards the progression of diabetic nephropathy, whereas hyporeninemia is known as a typical state in diabetic subjects. The purpose of this study is to determine whether expression levels of RAS differ between nondiabetic and diabetic renal tissues with accurate quantitative method. RESEARCH DESIGN AND METHODS - Subjects were 66 nondiabetic and 8 diabetic patients with biopsy-proven renal diseases. The eight diabetic subjects suffered from type 2 diabetes with overt proteinuria. Renal histology revealed typical diffuse or nodular lesions with linear IgG deposit on immunofluorescent staining and thickened basement membrane on electronic microscopy. Total RNA from a small part of the renal cortical biopsy specimens was reverse-transcribed, and the resultant cDNA was amplified for new major components of RAS (i.e., renin, renin receptor, angiotensinogen, ACE, ACE2, angiotensin II type 1 receptor, and angiotensin II type 2 receptor) and measured. RESULTS - Among these components, a significant upregulation was observed in the ACE gene in diabetic renal tissue. CONCLUSIONS - The results suggest that renal tissue RAS might be activated in the respect that ACE gene expression is upregulated in spite of a tendency to low renin expression in type 2 diabetic nephropathy.
AB - OBJECTIVE - Recent studies have proved that blockade of the renin-angiotensin system (RAS) retards the progression of diabetic nephropathy, whereas hyporeninemia is known as a typical state in diabetic subjects. The purpose of this study is to determine whether expression levels of RAS differ between nondiabetic and diabetic renal tissues with accurate quantitative method. RESEARCH DESIGN AND METHODS - Subjects were 66 nondiabetic and 8 diabetic patients with biopsy-proven renal diseases. The eight diabetic subjects suffered from type 2 diabetes with overt proteinuria. Renal histology revealed typical diffuse or nodular lesions with linear IgG deposit on immunofluorescent staining and thickened basement membrane on electronic microscopy. Total RNA from a small part of the renal cortical biopsy specimens was reverse-transcribed, and the resultant cDNA was amplified for new major components of RAS (i.e., renin, renin receptor, angiotensinogen, ACE, ACE2, angiotensin II type 1 receptor, and angiotensin II type 2 receptor) and measured. RESULTS - Among these components, a significant upregulation was observed in the ACE gene in diabetic renal tissue. CONCLUSIONS - The results suggest that renal tissue RAS might be activated in the respect that ACE gene expression is upregulated in spite of a tendency to low renin expression in type 2 diabetic nephropathy.
UR - http://www.scopus.com/inward/record.url?scp=33746368725&partnerID=8YFLogxK
U2 - 10.2337/diacare.29.04.06.dc05-1873
DO - 10.2337/diacare.29.04.06.dc05-1873
M3 - 記事
C2 - 16567826
AN - SCOPUS:33746368725
SN - 0149-5992
VL - 29
SP - 848
EP - 852
JO - Diabetes Care
JF - Diabetes Care
IS - 4
ER -
