Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling

Yoko Endo; Masashi Suzuki; Hideomi Yamada; Shoko Horita; Motoei Kunimi; Osamu Yamazaki; Ayumi Shirai; Motonobu Nakamura; Naoyuki Iso-O; Yuehong Li; Masumi Hara; Kazuhisa Tsukamoto; Nobuo Moriyama; Akihiko Kudo; Hayato Kawakami; Toshimasa Yamauchi; Naoto Kubota; Takashi Kadowaki; Haruki Kume; Yutaka Enomoto; Yukio Homma; George Seki; Toshiro Fujita

doi:10.1016/j.cmet.2011.02.015

Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling

Yoko Endo, Masashi Suzuki, Hideomi Yamada, Shoko Horita, Motoei Kunimi, Osamu Yamazaki, Ayumi Shirai, Motonobu Nakamura, Naoyuki Iso-O, Yuehong Li, Masumi Hara, Kazuhisa Tsukamoto, Nobuo Moriyama, Akihiko Kudo, Hayato Kawakami, Toshimasa Yamauchi, Naoto Kubota, Takashi Kadowaki, Haruki Kume, Yutaka EnomotoYukio Homma, George Seki, Toshiro Fujita

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Thiazolidinediones (TZDs) improve insulin resistance by activating a nuclear hormone receptor, peroxisome proliferator-activated receptor γ (PPARγ). However, the use of TZDs is associated with plasma volume expansion through a mechanism that remains to be clarified. Here we showed that TZDs rapidly stimulate sodium-coupled bicarbonate absorption from the renal proximal tubule in vitro and in vivo. TZD-induced transport stimulation is dependent on PPARγ-Src-EGFR-ERK and observed in rat, rabbit and human, but not in mouse proximal tubules where Src-EGFR is constitutively activated. The existence of PPARγ-Src-dependent nongenomic signaling, which requires the ligand-binding ability, but not the transcriptional activity of PPARγ, is confirmed in mouse embryonic fibroblast cells. The enhancement of the association between PPARγ and Src by TZDs supports an indispensable role of Src in this signaling. These results suggest that the PPARγ-dependent nongenomic stimulation of renal proximal transport is also involved in TZD-induced volume expansion.

Original language	English
Pages (from-to)	550-561
Number of pages	12
Journal	Cell Metabolism
Volume	13
Issue number	5
DOIs	https://doi.org/10.1016/j.cmet.2011.02.015
State	Published - 4 May 2011
Externally published	Yes

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Endo, Y., Suzuki, M., Yamada, H., Horita, S., Kunimi, M., Yamazaki, O., Shirai, A., Nakamura, M., Iso-O, N., Li, Y., Hara, M., Tsukamoto, K., Moriyama, N., Kudo, A., Kawakami, H., Yamauchi, T., Kubota, N., Kadowaki, T., Kume, H., ... Fujita, T. (2011). Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling. Cell Metabolism, 13(5), 550-561. https://doi.org/10.1016/j.cmet.2011.02.015

@article{f7e2ddaed7784777aa4ca4803b022bd7,

title = "Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling",

abstract = "Thiazolidinediones (TZDs) improve insulin resistance by activating a nuclear hormone receptor, peroxisome proliferator-activated receptor γ (PPARγ). However, the use of TZDs is associated with plasma volume expansion through a mechanism that remains to be clarified. Here we showed that TZDs rapidly stimulate sodium-coupled bicarbonate absorption from the renal proximal tubule in vitro and in vivo. TZD-induced transport stimulation is dependent on PPARγ-Src-EGFR-ERK and observed in rat, rabbit and human, but not in mouse proximal tubules where Src-EGFR is constitutively activated. The existence of PPARγ-Src-dependent nongenomic signaling, which requires the ligand-binding ability, but not the transcriptional activity of PPARγ, is confirmed in mouse embryonic fibroblast cells. The enhancement of the association between PPARγ and Src by TZDs supports an indispensable role of Src in this signaling. These results suggest that the PPARγ-dependent nongenomic stimulation of renal proximal transport is also involved in TZD-induced volume expansion.",

author = "Yoko Endo and Masashi Suzuki and Hideomi Yamada and Shoko Horita and Motoei Kunimi and Osamu Yamazaki and Ayumi Shirai and Motonobu Nakamura and Naoyuki Iso-O and Yuehong Li and Masumi Hara and Kazuhisa Tsukamoto and Nobuo Moriyama and Akihiko Kudo and Hayato Kawakami and Toshimasa Yamauchi and Naoto Kubota and Takashi Kadowaki and Haruki Kume and Yutaka Enomoto and Yukio Homma and George Seki and Toshiro Fujita",

year = "2011",

month = may,

day = "4",

doi = "10.1016/j.cmet.2011.02.015",

language = "英語",

volume = "13",

pages = "550--561",

journal = "Cell Metabolism",

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publisher = "Cell Press",

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Endo, Y, Suzuki, M, Yamada, H, Horita, S, Kunimi, M, Yamazaki, O, Shirai, A, Nakamura, M, Iso-O, N, Li, Y, Hara, M , Tsukamoto, K, Moriyama, N, Kudo, A, Kawakami, H, Yamauchi, T, Kubota, N, Kadowaki, T, Kume, H, Enomoto, Y, Homma, Y, Seki, G & Fujita, T 2011, 'Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling', Cell Metabolism, vol. 13, no. 5, pp. 550-561. https://doi.org/10.1016/j.cmet.2011.02.015

TY - JOUR

T1 - Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling

AU - Endo, Yoko

AU - Suzuki, Masashi

AU - Yamada, Hideomi

AU - Horita, Shoko

AU - Kunimi, Motoei

AU - Yamazaki, Osamu

AU - Shirai, Ayumi

AU - Nakamura, Motonobu

AU - Iso-O, Naoyuki

AU - Li, Yuehong

AU - Hara, Masumi

AU - Tsukamoto, Kazuhisa

AU - Moriyama, Nobuo

AU - Kudo, Akihiko

AU - Kawakami, Hayato

AU - Yamauchi, Toshimasa

AU - Kubota, Naoto

AU - Kadowaki, Takashi

AU - Kume, Haruki

AU - Enomoto, Yutaka

AU - Homma, Yukio

AU - Seki, George

AU - Fujita, Toshiro

PY - 2011/5/4

Y1 - 2011/5/4

N2 - Thiazolidinediones (TZDs) improve insulin resistance by activating a nuclear hormone receptor, peroxisome proliferator-activated receptor γ (PPARγ). However, the use of TZDs is associated with plasma volume expansion through a mechanism that remains to be clarified. Here we showed that TZDs rapidly stimulate sodium-coupled bicarbonate absorption from the renal proximal tubule in vitro and in vivo. TZD-induced transport stimulation is dependent on PPARγ-Src-EGFR-ERK and observed in rat, rabbit and human, but not in mouse proximal tubules where Src-EGFR is constitutively activated. The existence of PPARγ-Src-dependent nongenomic signaling, which requires the ligand-binding ability, but not the transcriptional activity of PPARγ, is confirmed in mouse embryonic fibroblast cells. The enhancement of the association between PPARγ and Src by TZDs supports an indispensable role of Src in this signaling. These results suggest that the PPARγ-dependent nongenomic stimulation of renal proximal transport is also involved in TZD-induced volume expansion.

AB - Thiazolidinediones (TZDs) improve insulin resistance by activating a nuclear hormone receptor, peroxisome proliferator-activated receptor γ (PPARγ). However, the use of TZDs is associated with plasma volume expansion through a mechanism that remains to be clarified. Here we showed that TZDs rapidly stimulate sodium-coupled bicarbonate absorption from the renal proximal tubule in vitro and in vivo. TZD-induced transport stimulation is dependent on PPARγ-Src-EGFR-ERK and observed in rat, rabbit and human, but not in mouse proximal tubules where Src-EGFR is constitutively activated. The existence of PPARγ-Src-dependent nongenomic signaling, which requires the ligand-binding ability, but not the transcriptional activity of PPARγ, is confirmed in mouse embryonic fibroblast cells. The enhancement of the association between PPARγ and Src by TZDs supports an indispensable role of Src in this signaling. These results suggest that the PPARγ-dependent nongenomic stimulation of renal proximal transport is also involved in TZD-induced volume expansion.

UR - http://www.scopus.com/inward/record.url?scp=79955624781&partnerID=8YFLogxK

U2 - 10.1016/j.cmet.2011.02.015

DO - 10.1016/j.cmet.2011.02.015

M3 - 記事

C2 - 21531337

AN - SCOPUS:79955624781

SN - 1550-4131

VL - 13

SP - 550

EP - 561

JO - Cell Metabolism

JF - Cell Metabolism

IS - 5

ER -

Thiazolidinediones enhance sodium-coupled bicarbonate absorption from renal proximal tubules via PPARγ-dependent nongenomic signaling

Abstract

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