Significance of a carboxyl-terminal amide moiety in the folding and biological activity of crustacean hyperglycemic hormone

Hidekazu Katayama; Tsuyoshi Ohira; Katsumi Aida; Hiromichi Nagasawa

doi:10.1016/S0196-9781(02)00094-3

Significance of a carboxyl-terminal amide moiety in the folding and biological activity of crustacean hyperglycemic hormone

Hidekazu Katayama, Tsuyoshi Ohira, Katsumi Aida, Hiromichi Nagasawa

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

86 Scopus citations

Abstract

Recombinant peptides related to Pej-SGP-I, one of several crustacean hyperglycemic hormones (CHHs) existing in the kuruma prawn Penaeus japonicus, were expressed in bacterial cells, and then purified after being allowed to refold. Their circular dichroism spectra suggested that the recombinant Pej-SGP-I having a free carboxyl-terminus (rPej-SGP-I-OH) differed slightly in secondary structure from the recombinant Pej-SGP-I having an amidated C-terminus (rPej-SGP-I-amide). The hyperglycemic activity of rPej-SGP-I-amide was comparable to that of natural Pej-SGP-I, whereas rPej-SGP-I-OH showed weaker hyperglycemic activity by approximately one order of magnitude. These results indicate that the C-terminal amide of CHH affects secondary structure and is significant in conferring hyperglycemic activity.

Original language	English
Pages (from-to)	1537-1546
Number of pages	10
Journal	Peptides
Volume	23
Issue number	9
DOIs	https://doi.org/10.1016/S0196-9781(02)00094-3
State	Published - Sep 2002
Externally published	Yes

Keywords

Carboxyl-terminal amide
Circular dichroism spectrum
Crustacean hyperglycemic hormone
Kuruma prawn
Penaeus japonicus
Secondary structure

Access to Document

10.1016/S0196-9781(02)00094-3

Cite this

@article{c470f871f7db4b87bcaad4e057513b47,

title = "Significance of a carboxyl-terminal amide moiety in the folding and biological activity of crustacean hyperglycemic hormone",

abstract = "Recombinant peptides related to Pej-SGP-I, one of several crustacean hyperglycemic hormones (CHHs) existing in the kuruma prawn Penaeus japonicus, were expressed in bacterial cells, and then purified after being allowed to refold. Their circular dichroism spectra suggested that the recombinant Pej-SGP-I having a free carboxyl-terminus (rPej-SGP-I-OH) differed slightly in secondary structure from the recombinant Pej-SGP-I having an amidated C-terminus (rPej-SGP-I-amide). The hyperglycemic activity of rPej-SGP-I-amide was comparable to that of natural Pej-SGP-I, whereas rPej-SGP-I-OH showed weaker hyperglycemic activity by approximately one order of magnitude. These results indicate that the C-terminal amide of CHH affects secondary structure and is significant in conferring hyperglycemic activity.",

keywords = "Carboxyl-terminal amide, Circular dichroism spectrum, Crustacean hyperglycemic hormone, Kuruma prawn, Penaeus japonicus, Secondary structure",

author = "Hidekazu Katayama and Tsuyoshi Ohira and Katsumi Aida and Hiromichi Nagasawa",

year = "2002",

month = sep,

doi = "10.1016/S0196-9781(02)00094-3",

language = "英語",

volume = "23",

pages = "1537--1546",

journal = "Peptides",

issn = "0196-9781",

publisher = "Elsevier Inc.",

number = "9",

}

TY - JOUR

T1 - Significance of a carboxyl-terminal amide moiety in the folding and biological activity of crustacean hyperglycemic hormone

AU - Katayama, Hidekazu

AU - Ohira, Tsuyoshi

AU - Aida, Katsumi

AU - Nagasawa, Hiromichi

PY - 2002/9

Y1 - 2002/9

N2 - Recombinant peptides related to Pej-SGP-I, one of several crustacean hyperglycemic hormones (CHHs) existing in the kuruma prawn Penaeus japonicus, were expressed in bacterial cells, and then purified after being allowed to refold. Their circular dichroism spectra suggested that the recombinant Pej-SGP-I having a free carboxyl-terminus (rPej-SGP-I-OH) differed slightly in secondary structure from the recombinant Pej-SGP-I having an amidated C-terminus (rPej-SGP-I-amide). The hyperglycemic activity of rPej-SGP-I-amide was comparable to that of natural Pej-SGP-I, whereas rPej-SGP-I-OH showed weaker hyperglycemic activity by approximately one order of magnitude. These results indicate that the C-terminal amide of CHH affects secondary structure and is significant in conferring hyperglycemic activity.

AB - Recombinant peptides related to Pej-SGP-I, one of several crustacean hyperglycemic hormones (CHHs) existing in the kuruma prawn Penaeus japonicus, were expressed in bacterial cells, and then purified after being allowed to refold. Their circular dichroism spectra suggested that the recombinant Pej-SGP-I having a free carboxyl-terminus (rPej-SGP-I-OH) differed slightly in secondary structure from the recombinant Pej-SGP-I having an amidated C-terminus (rPej-SGP-I-amide). The hyperglycemic activity of rPej-SGP-I-amide was comparable to that of natural Pej-SGP-I, whereas rPej-SGP-I-OH showed weaker hyperglycemic activity by approximately one order of magnitude. These results indicate that the C-terminal amide of CHH affects secondary structure and is significant in conferring hyperglycemic activity.

KW - Carboxyl-terminal amide

KW - Circular dichroism spectrum

KW - Crustacean hyperglycemic hormone

KW - Kuruma prawn

KW - Penaeus japonicus

KW - Secondary structure

UR - http://www.scopus.com/inward/record.url?scp=0036710663&partnerID=8YFLogxK

U2 - 10.1016/S0196-9781(02)00094-3

DO - 10.1016/S0196-9781(02)00094-3

M3 - 記事

C2 - 12217413

AN - SCOPUS:0036710663

SN - 0196-9781

VL - 23

SP - 1537

EP - 1546

JO - Peptides

JF - Peptides

IS - 9

ER -

Significance of a carboxyl-terminal amide moiety in the folding and biological activity of crustacean hyperglycemic hormone

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this