Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation

Hitomi Furuya; Ayumi Taguchi; Kei Kawana; Aki Yamashita; Eri Inoue; Mitsuyo Yoshida; Hiroe Nakamura; Asaha Fujimoto; Tomoko Inoue; Masakazu Sato; Haruka Nishida; Kazunori Nagasaka; Katsuyuki Adachi; Mari Hoya; Takeshi Nagamatsu; Osamu Wada-Hiraike; Takahiro Yamashita; Yutaka Osuga; Tomoyuki Fujii

doi:10.1177/1933719115589413

Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation

Hitomi Furuya, Ayumi Taguchi, Kei Kawana, Aki Yamashita, Eri Inoue, Mitsuyo Yoshida, Hiroe Nakamura, Asaha Fujimoto, Tomoko Inoue, Masakazu Sato, Haruka Nishida, Kazunori Nagasaka, Katsuyuki Adachi, Mari Hoya, Takeshi Nagamatsu, Osamu Wada-Hiraike, Takahiro Yamashita, Yutaka Osuga, Tomoyuki Fujii

The University of Tokyo

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Inflammatory cytokines play a major role in spontaneous preterm birth. Resveratrol has strong anti-inflammatory effects, but its effect on preterm birth in vivo is unknown. We investigated whether resveratrol protects against preterm birth in the lipopolysaccharide (LPS)-induced preterm mouse model. Twelve-day-old pregnant mice were fed 20 to 40 mg/kg resveratrol daily. On day 15, 10 μg of LPS was injected into uterine cervices. Resveratrol administration significantly decreased the rate of preterm birth. Resveratrol administration abolished LPS-induced elevation of tumor necrosis factor α (TNF-α) and interleukin (IL) 1β but not IL-6 levels. The TNF-α messenger RNA levels were decreased in the cervices of resveratrol-administered mice compared with controls. Resveratrol treatment suppressed the elevation in TNF-α and IL-1β levels in LPS-exposed peritoneal macrophages. Further resveratrol treatment eradicated the proinflammatory cytokine-mediated elevation in cyclooxygenase 2 (COX-2) in peritoneal macrophages. Resveratrol may protect against pathological preterm birth by suppression of elevated proinflammatory cytokines and consequent elevation of COX-2 in macrophages.

Original language	English
Pages (from-to)	1561-1568
Number of pages	8
Journal	Reproductive Sciences
Volume	22
Issue number	12
DOIs	https://doi.org/10.1177/1933719115589413
State	Published - 1 Dec 2015
Externally published	Yes

Keywords

anti-inflammatory effect
COX-2
LPS-induced preterm birth
peritoneal macrophage
resveratrol

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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Furuya, H., Taguchi, A., Kawana, K., Yamashita, A., Inoue, E., Yoshida, M., Nakamura, H., Fujimoto, A., Inoue, T., Sato, M., Nishida, H., Nagasaka, K., Adachi, K., Hoya, M., Nagamatsu, T., Wada-Hiraike, O., Yamashita, T., Osuga, Y., & Fujii, T. (2015). Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation. Reproductive Sciences, 22(12), 1561-1568. https://doi.org/10.1177/1933719115589413

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title = "Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation",

abstract = "Inflammatory cytokines play a major role in spontaneous preterm birth. Resveratrol has strong anti-inflammatory effects, but its effect on preterm birth in vivo is unknown. We investigated whether resveratrol protects against preterm birth in the lipopolysaccharide (LPS)-induced preterm mouse model. Twelve-day-old pregnant mice were fed 20 to 40 mg/kg resveratrol daily. On day 15, 10 μg of LPS was injected into uterine cervices. Resveratrol administration significantly decreased the rate of preterm birth. Resveratrol administration abolished LPS-induced elevation of tumor necrosis factor α (TNF-α) and interleukin (IL) 1β but not IL-6 levels. The TNF-α messenger RNA levels were decreased in the cervices of resveratrol-administered mice compared with controls. Resveratrol treatment suppressed the elevation in TNF-α and IL-1β levels in LPS-exposed peritoneal macrophages. Further resveratrol treatment eradicated the proinflammatory cytokine-mediated elevation in cyclooxygenase 2 (COX-2) in peritoneal macrophages. Resveratrol may protect against pathological preterm birth by suppression of elevated proinflammatory cytokines and consequent elevation of COX-2 in macrophages.",

keywords = "anti-inflammatory effect, COX-2, LPS-induced preterm birth, peritoneal macrophage, resveratrol",

author = "Hitomi Furuya and Ayumi Taguchi and Kei Kawana and Aki Yamashita and Eri Inoue and Mitsuyo Yoshida and Hiroe Nakamura and Asaha Fujimoto and Tomoko Inoue and Masakazu Sato and Haruka Nishida and Kazunori Nagasaka and Katsuyuki Adachi and Mari Hoya and Takeshi Nagamatsu and Osamu Wada-Hiraike and Takahiro Yamashita and Yutaka Osuga and Tomoyuki Fujii",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2015.",

year = "2015",

month = dec,

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doi = "10.1177/1933719115589413",

language = "英語",

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Furuya, H, Taguchi, A, Kawana, K, Yamashita, A, Inoue, E, Yoshida, M, Nakamura, H, Fujimoto, A, Inoue, T, Sato, M, Nishida, H , Nagasaka, K , Adachi, K, Hoya, M, Nagamatsu, T, Wada-Hiraike, O, Yamashita, T, Osuga, Y & Fujii, T 2015, 'Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation', Reproductive Sciences, vol. 22, no. 12, pp. 1561-1568. https://doi.org/10.1177/1933719115589413

TY - JOUR

T1 - Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation

AU - Furuya, Hitomi

AU - Taguchi, Ayumi

AU - Kawana, Kei

AU - Yamashita, Aki

AU - Inoue, Eri

AU - Yoshida, Mitsuyo

AU - Nakamura, Hiroe

AU - Fujimoto, Asaha

AU - Inoue, Tomoko

AU - Sato, Masakazu

AU - Nishida, Haruka

AU - Nagasaka, Kazunori

AU - Adachi, Katsuyuki

AU - Hoya, Mari

AU - Nagamatsu, Takeshi

AU - Wada-Hiraike, Osamu

AU - Yamashita, Takahiro

AU - Osuga, Yutaka

AU - Fujii, Tomoyuki

PY - 2015/12/1

Y1 - 2015/12/1

N2 - Inflammatory cytokines play a major role in spontaneous preterm birth. Resveratrol has strong anti-inflammatory effects, but its effect on preterm birth in vivo is unknown. We investigated whether resveratrol protects against preterm birth in the lipopolysaccharide (LPS)-induced preterm mouse model. Twelve-day-old pregnant mice were fed 20 to 40 mg/kg resveratrol daily. On day 15, 10 μg of LPS was injected into uterine cervices. Resveratrol administration significantly decreased the rate of preterm birth. Resveratrol administration abolished LPS-induced elevation of tumor necrosis factor α (TNF-α) and interleukin (IL) 1β but not IL-6 levels. The TNF-α messenger RNA levels were decreased in the cervices of resveratrol-administered mice compared with controls. Resveratrol treatment suppressed the elevation in TNF-α and IL-1β levels in LPS-exposed peritoneal macrophages. Further resveratrol treatment eradicated the proinflammatory cytokine-mediated elevation in cyclooxygenase 2 (COX-2) in peritoneal macrophages. Resveratrol may protect against pathological preterm birth by suppression of elevated proinflammatory cytokines and consequent elevation of COX-2 in macrophages.

AB - Inflammatory cytokines play a major role in spontaneous preterm birth. Resveratrol has strong anti-inflammatory effects, but its effect on preterm birth in vivo is unknown. We investigated whether resveratrol protects against preterm birth in the lipopolysaccharide (LPS)-induced preterm mouse model. Twelve-day-old pregnant mice were fed 20 to 40 mg/kg resveratrol daily. On day 15, 10 μg of LPS was injected into uterine cervices. Resveratrol administration significantly decreased the rate of preterm birth. Resveratrol administration abolished LPS-induced elevation of tumor necrosis factor α (TNF-α) and interleukin (IL) 1β but not IL-6 levels. The TNF-α messenger RNA levels were decreased in the cervices of resveratrol-administered mice compared with controls. Resveratrol treatment suppressed the elevation in TNF-α and IL-1β levels in LPS-exposed peritoneal macrophages. Further resveratrol treatment eradicated the proinflammatory cytokine-mediated elevation in cyclooxygenase 2 (COX-2) in peritoneal macrophages. Resveratrol may protect against pathological preterm birth by suppression of elevated proinflammatory cytokines and consequent elevation of COX-2 in macrophages.

KW - anti-inflammatory effect

KW - COX-2

KW - LPS-induced preterm birth

KW - peritoneal macrophage

KW - resveratrol

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U2 - 10.1177/1933719115589413

DO - 10.1177/1933719115589413

M3 - 記事

C2 - 26037299

AN - SCOPUS:84947060949

SN - 1933-7191

VL - 22

SP - 1561

EP - 1568

JO - Reproductive Sciences

JF - Reproductive Sciences

IS - 12

ER -

Resveratrol Protects Against Pathological Preterm Birth by Suppression of Macrophage-Mediated Inflammation

Abstract

Keywords

UN SDGs

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