Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

It is a challenging task to quantify mucin using conventional protein quantification methods due to the large number of glycans attached to the peptide, which make up approximately 50–90% of its molecular weight. To address this issue, we propose a simple quantification method that involves spotting mucins onto a membrane and staining them with Alcian blue.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages119-124
Number of pages6
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2763
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Alcian blue stain
  • Densitometry
  • Mucin
  • Polyvinylidene fluoride membranes
  • Quantitation

Fingerprint

Dive into the research topics of 'Quantitation of Mucin by Densitometry of an Alcian Blue-Stained Membrane'. Together they form a unique fingerprint.

Cite this