Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

Hidekazu Katayama; Kayo Nozawa; Osamu Nureki; Yoshiaki Nakahara; Hironobu Hojo

doi:10.1271/bbb.110653

Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

Hidekazu Katayama, Kayo Nozawa, Osamu Nureki, Yoshiaki Nakahara, Hironobu Hojo

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Pyrrolysine-tRNA ^Pyl complex is produced by pyrrolysyl- tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA ^Pyl in vitro. In addition, the PylRS/ tRNA ^Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA ^Pyl pair can be used in protein engineering technology.

Original language	English
Pages (from-to)	205-208
Number of pages	4
Journal	Bioscience, Biotechnology and Biochemistry
Volume	76
Issue number	1
DOIs	https://doi.org/10.1271/bbb.110653
State	Published - 2012
Externally published	Yes

Keywords

Desulfitobacterium hafnience
Pyrrolysine
Pyrrolysyl-tRNA synthetase

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10.1271/bbb.110653

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title = "Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo",

abstract = "Pyrrolysine-tRNA Pyl complex is produced by pyrrolysyl- tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA Pyl in vitro. In addition, the PylRS/ tRNA Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA Pyl pair can be used in protein engineering technology.",

keywords = "Desulfitobacterium hafnience, Pyrrolysine, Pyrrolysyl-tRNA synthetase",

author = "Hidekazu Katayama and Kayo Nozawa and Osamu Nureki and Yoshiaki Nakahara and Hironobu Hojo",

year = "2012",

doi = "10.1271/bbb.110653",

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pages = "205--208",

journal = "Bioscience, Biotechnology and Biochemistry",

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T1 - Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

AU - Katayama, Hidekazu

AU - Nozawa, Kayo

AU - Nureki, Osamu

AU - Nakahara, Yoshiaki

AU - Hojo, Hironobu

PY - 2012

Y1 - 2012

N2 - Pyrrolysine-tRNA Pyl complex is produced by pyrrolysyl- tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA Pyl in vitro. In addition, the PylRS/ tRNA Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA Pyl pair can be used in protein engineering technology.

AB - Pyrrolysine-tRNA Pyl complex is produced by pyrrolysyl- tRNA synthetase (PylRS). In this study, we investigated the substrate specificity of Desulfitobacterium hafnience PylRS. PylRS incorporated various L-lysine derivatives into tRNA Pyl in vitro. In addition, the PylRS/ tRNA Pyl pair introduced these lysine derivatives into the recombinant protein by the Escherichia coli expression system, indicating that this PylRS/tRNA Pyl pair can be used in protein engineering technology.

KW - Desulfitobacterium hafnience

KW - Pyrrolysine

KW - Pyrrolysyl-tRNA synthetase

UR - https://www.scopus.com/pages/publications/84856596556

U2 - 10.1271/bbb.110653

DO - 10.1271/bbb.110653

M3 - 記事

C2 - 22232266

AN - SCOPUS:84856596556

SN - 0916-8451

VL - 76

SP - 205

EP - 208

JO - Bioscience, Biotechnology and Biochemistry

JF - Bioscience, Biotechnology and Biochemistry

IS - 1

ER -

Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo

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Keywords

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