TY - JOUR
T1 - Properties of growth and molecular profiles of rat progenitor cells from ciliary epithelium
AU - Yanagi, Yasuo
AU - Inoue, Yuji
AU - Kawase, Yoko
AU - Uchida, Saiko
AU - Tamaki, Yasuhiro
AU - Araie, Makoto
AU - Okochi, Hitoshi
PY - 2006/3
Y1 - 2006/3
N2 - Recent studies have demonstrated that multipotent retinal stem or progenitor cells can be isolated from the ciliary epithelium (CE) of the eye using a neurosphere culture. In this study, we investigated the properties of growth and differentiation, and molecular profiles of rat adult ciliary epithelium (CE)-derived retinal progenitors and forebrain (FB) derived neurospheres. Under clonogenic culture conditions, we found that the CE-derived neurospheres contained fewer undifferentiated cells compared with the FB-derived neurospheres, and that CE-derived neurospheres initially expressed the set of Notch pathway molecules genes including Notch 1 and Delta 1, HES-1 and HES-5, but partially lose their expression after passaging. Furthermore, we found that the CE-derived neurospheres did not express several markers for in vivo embryonic retinal progenitors. Additionally, when the eye was divided into four subregions along its dorsoventral and nasotemporal axes and progenitor cells were obtained from the subregions, the progenitor cells did not express the subregion specific transcription factors, suggesting that subregional specificity is not maintained in vitro. Together, our results demonstrate that CE-derived progenitor cells may have intrinsic limitations in the production of cell types.
AB - Recent studies have demonstrated that multipotent retinal stem or progenitor cells can be isolated from the ciliary epithelium (CE) of the eye using a neurosphere culture. In this study, we investigated the properties of growth and differentiation, and molecular profiles of rat adult ciliary epithelium (CE)-derived retinal progenitors and forebrain (FB) derived neurospheres. Under clonogenic culture conditions, we found that the CE-derived neurospheres contained fewer undifferentiated cells compared with the FB-derived neurospheres, and that CE-derived neurospheres initially expressed the set of Notch pathway molecules genes including Notch 1 and Delta 1, HES-1 and HES-5, but partially lose their expression after passaging. Furthermore, we found that the CE-derived neurospheres did not express several markers for in vivo embryonic retinal progenitors. Additionally, when the eye was divided into four subregions along its dorsoventral and nasotemporal axes and progenitor cells were obtained from the subregions, the progenitor cells did not express the subregion specific transcription factors, suggesting that subregional specificity is not maintained in vitro. Together, our results demonstrate that CE-derived progenitor cells may have intrinsic limitations in the production of cell types.
KW - Ciliary epithelium
KW - Retinal cell culture
KW - Retinal cell differentiation
KW - Retinal cell lineages
UR - https://www.scopus.com/pages/publications/31344451676
U2 - 10.1016/j.exer.2005.08.005
DO - 10.1016/j.exer.2005.08.005
M3 - 記事
C2 - 16198338
AN - SCOPUS:31344451676
SN - 0014-4835
VL - 82
SP - 471
EP - 478
JO - Experimental Eye Research
JF - Experimental Eye Research
IS - 3
ER -