Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

Kaori Kanemaru; Makoto Shimozawa; Manabu Kitamata; Rikuto Furuishi; Hinako Kayano; Yui Sukawa; Yuuki Chiba; Takatsugu Fukuyama; Junya Hasegawa; Hiroki Nakanishi; Takuma Kishimoto; Kazuya Tsujita; Kazuma Tanaka; Toshiki Itoh; Junko Sasaki; Takehiko Sasaki; Kiyoko Fukami; Yoshikazu Nakamura

doi:10.1038/s41467-022-30061-9

Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

Kaori Kanemaru
, Makoto Shimozawa
, Manabu Kitamata
, Rikuto Furuishi
, Hinako Kayano
, Yui Sukawa
, Yuuki Chiba
, Takatsugu Fukuyama
, Junya Hasegawa
, Hiroki Nakanishi
, Takuma Kishimoto
, Kazuya Tsujita
, Kazuma Tanaka
, Toshiki Itoh
, Junko Sasaki
, Takehiko Sasaki
, Kiyoko Fukami
, Yoshikazu Nakamura

Advanced Comprehensive Research Organization

Research output: Contribution to journal › Article

22 Scopus citations

Abstract

Abstract Epithelial cells provide cell-cell adhesion that is essential to maintain the integrity of multicellular organisms. Epithelial cell-characterizing proteins, such as epithelial junctional proteins and transcription factors are well defined. However, the role of lipids in epithelial characterization remains poorly understood. Here we show that the phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P₂] is enriched in the plasma membrane (PM) of epithelial cells. Epithelial cells lose their characteristics upon depletion of PM PI(4,5)P₂, and synthesis of PI(4,5)P₂ in the PM results in the development of epithelial-like morphology in osteosarcoma cells. PM localization of PARD3 is impaired by depletion of PM PI(4,5)P₂ in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P₂ regulates epithelial characteristics by recruiting PARD3 to the PM. These results indicate that a high level of PM PI(4,5)P₂ plays a crucial role in the maintenance of epithelial characteristics.

Original language	English
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-30061-9
State	Published - 9 May 2022

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Access to Document

10.1038/s41467-022-30061-9License: Unspecified

Cite this

Kanemaru, K., Shimozawa, M., Kitamata, M., Furuishi, R., Kayano, H., Sukawa, Y., Chiba, Y., Fukuyama, T., Hasegawa, J., Nakanishi, H., Kishimoto, T., Tsujita, K., Tanaka, K., Itoh, T., Sasaki, J., Sasaki, T., Fukami, K., & Nakamura, Y. (2022). Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30061-9

@article{1428c5c5f939468eaa6fc2b3c74e9308,

title = "Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics",

abstract = "Abstract Epithelial cells provide cell-cell adhesion that is essential to maintain the integrity of multicellular organisms. Epithelial cell-characterizing proteins, such as epithelial junctional proteins and transcription factors are well defined. However, the role of lipids in epithelial characterization remains poorly understood. Here we show that the phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] is enriched in the plasma membrane (PM) of epithelial cells. Epithelial cells lose their characteristics upon depletion of PM PI(4,5)P2, and synthesis of PI(4,5)P2 in the PM results in the development of epithelial-like morphology in osteosarcoma cells. PM localization of PARD3 is impaired by depletion of PM PI(4,5)P2 in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P2 regulates epithelial characteristics by recruiting PARD3 to the PM. These results indicate that a high level of PM PI(4,5)P2 plays a crucial role in the maintenance of epithelial characteristics.",

author = "Kaori Kanemaru and Makoto Shimozawa and Manabu Kitamata and Rikuto Furuishi and Hinako Kayano and Yui Sukawa and Yuuki Chiba and Takatsugu Fukuyama and Junya Hasegawa and Hiroki Nakanishi and Takuma Kishimoto and Kazuya Tsujita and Kazuma Tanaka and Toshiki Itoh and Junko Sasaki and Takehiko Sasaki and Kiyoko Fukami and Yoshikazu Nakamura",

year = "2022",

month = may,

day = "9",

doi = "10.1038/s41467-022-30061-9",

language = "英語",

volume = "13",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

}

Kanemaru, K, Shimozawa, M, Kitamata, M, Furuishi, R, Kayano, H, Sukawa, Y, Chiba, Y, Fukuyama, T, Hasegawa, J, Nakanishi, H, Kishimoto, T, Tsujita, K, Tanaka, K, Itoh, T, Sasaki, J, Sasaki, T, Fukami, K & Nakamura, Y 2022, 'Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics', Nature Communications, vol. 13, no. 1. https://doi.org/10.1038/s41467-022-30061-9

TY - JOUR

T1 - Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

AU - Kanemaru, Kaori

AU - Shimozawa, Makoto

AU - Kitamata, Manabu

AU - Furuishi, Rikuto

AU - Kayano, Hinako

AU - Sukawa, Yui

AU - Chiba, Yuuki

AU - Fukuyama, Takatsugu

AU - Hasegawa, Junya

AU - Nakanishi, Hiroki

AU - Kishimoto, Takuma

AU - Tsujita, Kazuya

AU - Tanaka, Kazuma

AU - Itoh, Toshiki

AU - Sasaki, Junko

AU - Sasaki, Takehiko

AU - Fukami, Kiyoko

AU - Nakamura, Yoshikazu

PY - 2022/5/9

Y1 - 2022/5/9

N2 - Abstract Epithelial cells provide cell-cell adhesion that is essential to maintain the integrity of multicellular organisms. Epithelial cell-characterizing proteins, such as epithelial junctional proteins and transcription factors are well defined. However, the role of lipids in epithelial characterization remains poorly understood. Here we show that the phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] is enriched in the plasma membrane (PM) of epithelial cells. Epithelial cells lose their characteristics upon depletion of PM PI(4,5)P2, and synthesis of PI(4,5)P2 in the PM results in the development of epithelial-like morphology in osteosarcoma cells. PM localization of PARD3 is impaired by depletion of PM PI(4,5)P2 in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P2 regulates epithelial characteristics by recruiting PARD3 to the PM. These results indicate that a high level of PM PI(4,5)P2 plays a crucial role in the maintenance of epithelial characteristics.

AB - Abstract Epithelial cells provide cell-cell adhesion that is essential to maintain the integrity of multicellular organisms. Epithelial cell-characterizing proteins, such as epithelial junctional proteins and transcription factors are well defined. However, the role of lipids in epithelial characterization remains poorly understood. Here we show that the phospholipid phosphatidylinositol (4,5)-bisphosphate [PI(4,5)P2] is enriched in the plasma membrane (PM) of epithelial cells. Epithelial cells lose their characteristics upon depletion of PM PI(4,5)P2, and synthesis of PI(4,5)P2 in the PM results in the development of epithelial-like morphology in osteosarcoma cells. PM localization of PARD3 is impaired by depletion of PM PI(4,5)P2 in epithelial cells, whereas expression of the PM-targeting exocyst-docking region of PARD3 induces osteosarcoma cells to show epithelial-like morphological changes, suggesting that PI(4,5)P2 regulates epithelial characteristics by recruiting PARD3 to the PM. These results indicate that a high level of PM PI(4,5)P2 plays a crucial role in the maintenance of epithelial characteristics.

U2 - 10.1038/s41467-022-30061-9

DO - 10.1038/s41467-022-30061-9

M3 - 記事

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

Plasma membrane phosphatidylinositol (4,5)-bisphosphate is critical for determination of epithelial characteristics

Abstract

UN SDGs

Access to Document

Fingerprint

Cite this