Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells

Akiko Kubota; Rui Kano; Takuya Mizuno; Masaharu Hisasue; Peter F. Moore; Toshihiro Watari; Hajime Tsujimoto; Atsuhiko Hasegawa

doi:10.1292/jvms.64.835

Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells

Akiko Kubota, Rui Kano, Takuya Mizuno, Masaharu Hisasue, Peter F. Moore, Toshihiro Watari, Hajime Tsujimoto, Atsuhiko Hasegawa

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

Original language	English
Pages (from-to)	835-837
Number of pages	3
Journal	Journal of Veterinary Medical Science
Volume	64
Issue number	9
DOIs	https://doi.org/10.1292/jvms.64.835
State	Published - Sep 2002
Externally published	Yes

Keywords

Hypercalcemia
Lymphoma cell
mRNA
Parathyroid hormone-related protein
RT-PCR

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1292/jvms.64.835

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title = "Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells",

abstract = "Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.",

keywords = "Hypercalcemia, Lymphoma cell, mRNA, Parathyroid hormone-related protein, RT-PCR",

author = "Akiko Kubota and Rui Kano and Takuya Mizuno and Masaharu Hisasue and Moore, {Peter F.} and Toshihiro Watari and Hajime Tsujimoto and Atsuhiko Hasegawa",

year = "2002",

month = sep,

doi = "10.1292/jvms.64.835",

language = "英語",

volume = "64",

pages = "835--837",

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AU - Kubota, Akiko

AU - Kano, Rui

AU - Mizuno, Takuya

AU - Hisasue, Masaharu

AU - Moore, Peter F.

AU - Watari, Toshihiro

AU - Tsujimoto, Hajime

AU - Hasegawa, Atsuhiko

PY - 2002/9

Y1 - 2002/9

N2 - Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

AB - Parathyroid hormone-related protein (PTHrP) was investigated in a canine lymphoma case with hypercalcemia by means of immunoradiomentric assay (IRMA) and molecular analysis. The plasma calcium level of the patient dog was 13.7 mg/d/. The PTHrP concentration examined by IRMA was 6.1 pmol/L in the plasma sample from the dog, but it was undetectable (< 1.1 pmol/L) in plasma samples from 4 lymphoma cases without hypercalcemia or 5 normal dogs. The PTHrP concentration examined in the culture supernatant of the lymphoma cells from this case was 1.3 pmol/L, whereas those of the lymphoma cells from a lymphoma case without hypercalcemia was undetectable. PTHrP mRNA was clearly detected not only in the lymphoma cells from this dog with hypercalcemia but also in lymphoma cells from 4 lymphoma cases without hypercalcemia and 2 canine lymphoma cell lines.

KW - Hypercalcemia

KW - Lymphoma cell

KW - mRNA

KW - Parathyroid hormone-related protein

KW - RT-PCR

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DO - 10.1292/jvms.64.835

M3 - 記事

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SN - 0916-7250

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EP - 837

JO - Journal of Veterinary Medical Science

JF - Journal of Veterinary Medical Science

IS - 9

ER -

Parathyroid hormone-related protein (PTHrP) produced by dog lymphoma cells

Abstract

Keywords

UN SDGs

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