@inproceedings{0bfa5e21c81f45aebd75291f505158c8,
title = "Microfluidic perfusion culture of human induced pluripotent stem cell in microchamber array chip",
abstract = "This paper reports on the microfluidic perfusion culture of human induced pluripotent stem cells (hiPSCs) under fully defined culture condition. By screening various ECMs we determined that fibronectin and laminin are appropriate for microfluidic devices made out of the most popular material, polydimethylsiloxane (PDMS). We found that the growth rate of hiPSCs under pressure-driven perfusion culture conditions was higher than under static culture conditions in the microchamber array. We believe that our system will be a platform technology for future large-scale screening of fully defined conditions for differentiation cultures on integrated microfluidic devices.",
keywords = "Human induced pluripotent stem cells, Microchamber array, Perfusion culture",
author = "R. Yoshimitsu and K. Hattori and S. Sugiura and Y. Kondo and T. Satoh and A. Kurisaki and M. Asashima and K. Ohnuma and T. Kanamori",
year = "2013",
language = "英語",
isbn = "9781632666246",
series = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
publisher = "Chemical and Biological Microsystems Society",
pages = "1072--1074",
booktitle = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013",
note = "17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013 ; Conference date: 27-10-2013 Through 31-10-2013",
}