Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

Kotaro Horiguchi, Ken Fujiwara, Saishu Yoshida, Takashi Nakakura, Ken Arae, Takehiro Tsukada, Rumi Hasegawa, Shu Takigami, Shunji Ohsako, Takashi Yashiro, Takako Kato, Yukio Kato

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26 Scopus citations

Abstract

S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.

Original languageEnglish
Article number5533
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - 1 Dec 2018

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