Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

Kotaro Horiguchi; Ken Fujiwara; Saishu Yoshida; Takashi Nakakura; Ken Arae; Takehiro Tsukada; Rumi Hasegawa; Shu Takigami; Shunji Ohsako; Takashi Yashiro; Takako Kato; Yukio Kato

doi:10.1038/s41598-018-23923-0

Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

Kotaro Horiguchi, Ken Fujiwara, Saishu Yoshida, Takashi Nakakura, Ken Arae, Takehiro Tsukada, Rumi Hasegawa, Shu Takigami, Shunji Ohsako, Takashi Yashiro, Takako Kato, Yukio Kato

School of Medicine

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.

Original language	English
Article number	5533
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-23923-0
State	Published - 1 Dec 2018

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title = "Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats",

abstract = "S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.",

author = "Kotaro Horiguchi and Ken Fujiwara and Saishu Yoshida and Takashi Nakakura and Ken Arae and Takehiro Tsukada and Rumi Hasegawa and Shu Takigami and Shunji Ohsako and Takashi Yashiro and Takako Kato and Yukio Kato",

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doi = "10.1038/s41598-018-23923-0",

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AU - Horiguchi, Kotaro

AU - Fujiwara, Ken

AU - Yoshida, Saishu

AU - Nakakura, Takashi

AU - Arae, Ken

AU - Tsukada, Takehiro

AU - Hasegawa, Rumi

AU - Takigami, Shu

AU - Ohsako, Shunji

AU - Yashiro, Takashi

AU - Kato, Takako

AU - Kato, Yukio

PY - 2018/12/1

Y1 - 2018/12/1

N2 - S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.

AB - S100β protein and SOX2-double positive (S100β/SOX2-positive) cells have been suggested to be adult pituitary stem/progenitor cells exhibiting plasticity and multipotency. The aim of the present study was to isolate S100β/SOX2-positive cells from the adult anterior lobes of rats using a specific antibody against a novel membrane marker and to study their characteristics in vitro. We found that cluster of differentiation (CD) 9 is expressed in the majority of adult rat S100β/SOX2-positive cells, and we succeeded in isolating CD9-positive cells using an anti-CD9 antibody with a pluriBead-cascade cell isolation system. Cultivation of these cells showed their capacity to differentiate into endothelial cells via bone morphogenetic protein signalling. By using the anterior lobes of prolactinoma model rats, the localisation of CD9-positive cells was confirmed in the tumour-induced neovascularisation region. Thus, the present study provides novel insights into adult pituitary stem/progenitor cells involved in the vascularisation of the anterior lobe.

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SN - 2045-2322

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JO - Scientific Reports

JF - Scientific Reports

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ER -

Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats

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