In vivo evaluation of neutrophil recruitment in response to sterile particulates

Tamiko Yanagida, Kaoru Orihashi, Hajime Kono

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

2 Scopus citations

Abstract

Sterile particulates such as monosodium urate crystals induce inflammasome activation resulting in activation of caspase-1, secretion of IL-1α, and processing of IL-1β. Local production and activation of IL-1 leads to neutrophil recruitment in vivo. Here we describe two quick and simple methods for the evaluation of neutrophil recruitment in the peritoneal cavity and skin in response to sterile particulates, which are dependent on IL-1 receptor signaling.

Original languageEnglish
Title of host publicationThe Inflammasome
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages211-221
Number of pages11
ISBN (Print)9781627035224
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1040
ISSN (Print)1064-3745

Keywords

  • Acute inflammation
  • IL-1
  • Monocytes
  • Myeloperoxidase
  • Neutrophils
  • Peritoneum
  • Skin

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