In Vitro Replication Study of Modified Bases in ras Sequences

Hiroyuki Kamiya; Tomoki Sakaguchi; Naoko Murata; Hideo Inoue; Miho Shimizu; Eiko Ohtsuka; I. Ishikawa; Masahiro Fujimuro; Hiroyuki Miura; Susumu Nishimura; Akio Matsukage; Chikahide Masutani

doi:10.1248/cpb.40.2792

In Vitro Replication Study of Modified Bases in ras Sequences

Hiroyuki Kamiya, Tomoki Sakaguchi, Naoko Murata, Hideo Inoue, Miho Shimizu, Eiko Ohtsuka, I. Ishikawa, Masahiro Fujimuro, Hiroyuki Miura, Susumu Nishimura, Akio Matsukage, Chikahide Masutani

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

DNA templates containing a modified base (0⁶-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase β and mouse DNA polymerase a-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.

Original language	English
Pages (from-to)	2792-2795
Number of pages	4
Journal	Chemical and Pharmaceutical Bulletin
Volume	40
Issue number	10
DOIs	https://doi.org/10.1248/cpb.40.2792
State	Published - 1992
Externally published	Yes

Keywords

06-methylguanine
8-hydroxyguanine
DNA polymerase /?
DNA polymerase a
Taq DNA polymerase
hypoxanthine
ras gene
replication
xanthine

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10.1248/cpb.40.2792

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title = "In Vitro Replication Study of Modified Bases in ras Sequences",

abstract = "DNA templates containing a modified base (06-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase β and mouse DNA polymerase a-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.",

keywords = "06-methylguanine, 8-hydroxyguanine, DNA polymerase /?, DNA polymerase a, Taq DNA polymerase, hypoxanthine, ras gene, replication, xanthine",

author = "Hiroyuki Kamiya and Tomoki Sakaguchi and Naoko Murata and Hideo Inoue and Miho Shimizu and Eiko Ohtsuka and I. Ishikawa and Masahiro Fujimuro and Hiroyuki Miura and Susumu Nishimura and Akio Matsukage and Chikahide Masutani",

year = "1992",

doi = "10.1248/cpb.40.2792",

language = "英語",

volume = "40",

pages = "2792--2795",

journal = "Chemical and Pharmaceutical Bulletin",

issn = "0009-2363",

publisher = "Pharmaceutical Society of Japan",

number = "10",

}

TY - JOUR

T1 - In Vitro Replication Study of Modified Bases in ras Sequences

AU - Kamiya, Hiroyuki

AU - Sakaguchi, Tomoki

AU - Murata, Naoko

AU - Inoue, Hideo

AU - Shimizu, Miho

AU - Ohtsuka, Eiko

AU - Ishikawa, I.

AU - Fujimuro, Masahiro

AU - Miura, Hiroyuki

AU - Nishimura, Susumu

AU - Matsukage, Akio

AU - Masutani, Chikahide

PY - 1992

Y1 - 1992

N2 - DNA templates containing a modified base (06-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase β and mouse DNA polymerase a-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.

AB - DNA templates containing a modified base (06-methylguanine, 8-hydroxyguanine, xanthine or hypoxanthine) which was located in nucleotide sequences corresponding to the 12th or 61st codon of a ras gene were synthesized and deoxynucleotide incorporation opposite the lesions was investigated. The templates were replicated by Taq DNA polymerase, recombinant rat DNA polymerase β and mouse DNA polymerase a-primase complex. Sequence analysis of the replicated products indicated selective incorporation of nucleotide(s) opposite a modified base, depending on the kind of base and of DNA polymerase. This system is very useful to obtain results of in vitro replication of modified bases in ras sequences.

KW - 06-methylguanine

KW - 8-hydroxyguanine

KW - DNA polymerase /?

KW - DNA polymerase a

KW - Taq DNA polymerase

KW - hypoxanthine

KW - ras gene

KW - replication

KW - xanthine

UR - https://www.scopus.com/pages/publications/0026463470

U2 - 10.1248/cpb.40.2792

DO - 10.1248/cpb.40.2792

M3 - 記事

C2 - 1464111

AN - SCOPUS:0026463470

SN - 0009-2363

VL - 40

SP - 2792

EP - 2795

JO - Chemical and Pharmaceutical Bulletin

JF - Chemical and Pharmaceutical Bulletin

IS - 10

ER -

In Vitro Replication Study of Modified Bases in ras Sequences

Abstract

Keywords

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