Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray analysis

Tomoyuki Fukasawa; Koichiro Murashima; Tomoko Nemoto; Ichiro Matsumoto; Jinichiro Koga; Hidetoshi Kubota; Minoru Kanegae

doi:10.1080/19390210903070822

Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray analysis

Tomoyuki Fukasawa
, Koichiro Murashima
, Tomoko Nemoto
, Ichiro Matsumoto
, Jinichiro Koga
, Hidetoshi Kubota
, Minoru Kanegae

Meiji Holdings Co., Ltd.
The University of Tokyo

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p <.05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.

Original language	English
Pages (from-to)	254-262
Number of pages	9
Journal	Journal of Dietary Supplements
Volume	6
Issue number	3
DOIs	https://doi.org/10.1080/19390210903070822
State	Published - 2009
Externally published	Yes

Keywords

Lipid-lowering effect
Marker gene
Short-chain fructooligosaccharide

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10.1080/19390210903070822

Cite this

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abstract = "Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p <.05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.",

keywords = "Lipid-lowering effect, Marker gene, Short-chain fructooligosaccharide",

author = "Tomoyuki Fukasawa and Koichiro Murashima and Tomoko Nemoto and Ichiro Matsumoto and Jinichiro Koga and Hidetoshi Kubota and Minoru Kanegae",

year = "2009",

doi = "10.1080/19390210903070822",

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T1 - Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray analysis

AU - Fukasawa, Tomoyuki

AU - Murashima, Koichiro

AU - Nemoto, Tomoko

AU - Matsumoto, Ichiro

AU - Koga, Jinichiro

AU - Kubota, Hidetoshi

AU - Kanegae, Minoru

PY - 2009

Y1 - 2009

N2 - Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p <.05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.

AB - Administration of short-chain fructooligosaccharide (scFOS) is known to lower serum triglyceride levels in rats fed a high-fat diet, but the molecular mechanisms remain unclear. This study aimed to identify marker genes for lipid-lowering effect of scFOS administration. The changes in hepatic gene expressions in rats fed scFOS were investigated using DNA microarray and quantitative RT-PCR analysis. The DNA microarray showed that phytanoyl-CoA 2-hydroxylase 2 (Phyh2), lipoprotein lipase (Lpl) and tyrosine aminotransferase (Tat) were significantly affected by scFOS administration (p <.05). Since Lpl is involved in lipid metabolism, the up-regulation of Lpl in the liver can be a potential marker of the lipid-lowering effect of scFOS.

KW - Lipid-lowering effect

KW - Marker gene

KW - Short-chain fructooligosaccharide

UR - https://www.scopus.com/pages/publications/70349464490

U2 - 10.1080/19390210903070822

DO - 10.1080/19390210903070822

M3 - 記事

C2 - 22435477

AN - SCOPUS:70349464490

SN - 1939-0211

VL - 6

SP - 254

EP - 262

JO - Journal of Dietary Supplements

JF - Journal of Dietary Supplements

IS - 3

ER -

Identification of marker genes for lipid-lowering effect of a short-chain fructooligosaccharide by DNA microarray analysis

Abstract

Keywords

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