TY - JOUR
T1 - Gene expression of (pro)renin receptor is upregulated in hearts and kidneys of rats with congestive heart failure
AU - Hirose, Takuo
AU - Mori, Nobuyoshi
AU - Totsune, Kazuhito
AU - Morimoto, Ryo
AU - Maejima, Takahiro
AU - Kawamura, Takuya
AU - Metoki, Hirohito
AU - Asayama, Kei
AU - Kikuya, Masahiro
AU - Ohkubo, Takayoshi
AU - Kohzuki, Masahiro
AU - Takahashi, Kazuhiro
AU - Imai, Yutaka
PY - 2009/12
Y1 - 2009/12
N2 - Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure.
AB - Recent studies have revealed that (pro)renin receptor ((P)RR), a newly identified member of the renin-angiotensin system, was associated with organ damage in the kidney. However, there has been little information for (P)RR in hearts. To investigate the regulation of (P)RR in heart failure, we examined the expression of (P)RR in hearts and kidneys of rats with congestive heart failure (CHF) due to coronary ligation by quantitative RT-PCR and immunohistochemistry. Significantly increased levels of (P)RR mRNA were found in the atrium, right ventricle, non-infarcted part of left ventricle, infarcted part of left ventricle and kidney of CHF rats, when compared with sham operated rats (about 1.6-fold, 1.4-fold, 1.6-fold, 1.7-fold and 1.5-fold, respectively). Expression levels of mRNAs encoding renin and angiotensinogen in these heart and kidney tissues were also increased in the CHF rats. Immunohistochemistry showed positive (P)RR immunostaining in the myocardium, the renal tubular cells, and vascular smooth muscle and endothelial cells in the heart and the kidney. The renal tubular cells were more intensely immunostained in CHF rats than in sham operated rats. These findings suggest that the expression of (P)RR is increased in the hearts and kidneys of rats with heart failure, and that (P)RR may contribute to heart failure.
KW - Immunohistochemistry
KW - Organ damage
KW - Renin-angiotensin system
KW - RT-PCR
UR - http://www.scopus.com/inward/record.url?scp=70449646204&partnerID=8YFLogxK
U2 - 10.1016/j.peptides.2009.09.015
DO - 10.1016/j.peptides.2009.09.015
M3 - 記事
C2 - 19765626
AN - SCOPUS:70449646204
SN - 0196-9781
VL - 30
SP - 2316
EP - 2322
JO - Peptides
JF - Peptides
IS - 12
ER -
