Establishment of an enzyme-linked immunosorbent assay for detection of Hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli

Akira Takakura; Kazuo Goto; Toshio Itoh; Kumiko Yoshimatsu; Ikuo Takashima; Jiro Arikawa

doi:10.1538/expanim.52.25

Establishment of an enzyme-linked immunosorbent assay for detection of Hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli

Akira Takakura, Kazuo Goto, Toshio Itoh, Kumiko Yoshimatsu, Ikuo Takashima, Jiro Arikawa

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR- 11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.

Original language	English
Pages (from-to)	25-30
Number of pages	6
Journal	Experimental Animals
Volume	52
Issue number	1
DOIs	https://doi.org/10.1538/expanim.52.25
State	Published - 2003
Externally published	Yes

Keywords

ELISA
Hantavirus
Rats
Recombinant antigen

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1538/expanim.52.25

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title = "Establishment of an enzyme-linked immunosorbent assay for detection of Hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli",

abstract = "A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR- 11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.",

keywords = "ELISA, Hantavirus, Rats, Recombinant antigen",

author = "Akira Takakura and Kazuo Goto and Toshio Itoh and Kumiko Yoshimatsu and Ikuo Takashima and Jiro Arikawa",

year = "2003",

doi = "10.1538/expanim.52.25",

language = "英語",

volume = "52",

pages = "25--30",

journal = "Experimental Animals",

issn = "1341-1357",

publisher = "International Press Editing Centre Incorporation",

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TY - JOUR

T1 - Establishment of an enzyme-linked immunosorbent assay for detection of Hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli

AU - Takakura, Akira

AU - Goto, Kazuo

AU - Itoh, Toshio

AU - Yoshimatsu, Kumiko

AU - Takashima, Ikuo

AU - Arikawa, Jiro

PY - 2003

Y1 - 2003

N2 - A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR- 11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.

AB - A recombinant nucleocapsid protein of Hantaan virus (HTN) 76-118 strain expressed in E. coli was applied as a serodiagnostic antigen in an enzyme-linked immunosorbent assay (rHTN-ELISA) for detection of hantavirus antibody in rat sera. The sensitivity and specificity of the rHTN-ELISA were compared with those of the indirect immunofluoresent assay (IFA) using virus-infected cells. The sensitivity of rHTN-ELISA was similar to that of the IFA both in experimentally SR- 11 infected rat and naturally infected rat sera. Sera showing a low antibody titer in IFA and suspected to be negative by other methods were also found to be negative in rHTN-ELISA. These results indicate that rHTN-ELISA is effective as a screening method for serodiagnosis of hantaviruses, because of its high sensitivity, specificity, safety and suitability for processing large number of samples.

KW - ELISA

KW - Hantavirus

KW - Rats

KW - Recombinant antigen

UR - https://www.scopus.com/pages/publications/0038352161

U2 - 10.1538/expanim.52.25

DO - 10.1538/expanim.52.25

M3 - 記事

C2 - 12638233

AN - SCOPUS:0038352161

SN - 1341-1357

VL - 52

SP - 25

EP - 30

JO - Experimental Animals

JF - Experimental Animals

IS - 1

ER -

Establishment of an enzyme-linked immunosorbent assay for detection of Hantavirus antibody of rats using a recombinant of nucleocapsid protein expressed in Escherichia coli

Abstract

Keywords

UN SDGs

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