TY - JOUR
T1 - Effects of arachidonic acid on the metabolism of eicosapentaenoic acid in washed human platelets
AU - Hashimoto, Yoshiaki
AU - Naito, Chikayuki
AU - Teramoto, Tamio
AU - Kato, Hirokazu
AU - Kawamura, Mitsunobu
AU - Oka, Hiroshi
PY - 1985/11/1
Y1 - 1985/11/1
N2 - We examined effects of arachidonic acid (AA) on eicosapentaenoic acid (EPA) metabolism in washed human platelets. Although human platelets had been considered to metabolize scarcely EPA, a simultaneous addition of EPA and AA to washed platelet suspensions stimulated markedly EPA metabolism. In addition, the stimulatory effect was more potent over the formation of thromboxane (TX) B3 than that of 12-hydroxy-5,8,10,14,17-eicosapentaenoic acid (HEPE). The stimulation by AA can be due to AA itself and/or AA metabolites. Indomethacin decreased the stimulatory effect of AA on the HEPE formation, suggesting that cyclooxygenase product(s) of AA stimulated the HEPE formation. Among the metabolites of AA investigated, prostaglandin (PG)G2 and 12-hydroperoxy-5,8,10,14-eicosatetraenoic acid had the stimulatory effect on both TXB2 and HEPE formations, whereas PGH2, PGD2, TXB2 and 12-hydroxy-5, 8,10,14-eicosatetraenoic acid were ineffective.
AB - We examined effects of arachidonic acid (AA) on eicosapentaenoic acid (EPA) metabolism in washed human platelets. Although human platelets had been considered to metabolize scarcely EPA, a simultaneous addition of EPA and AA to washed platelet suspensions stimulated markedly EPA metabolism. In addition, the stimulatory effect was more potent over the formation of thromboxane (TX) B3 than that of 12-hydroxy-5,8,10,14,17-eicosapentaenoic acid (HEPE). The stimulation by AA can be due to AA itself and/or AA metabolites. Indomethacin decreased the stimulatory effect of AA on the HEPE formation, suggesting that cyclooxygenase product(s) of AA stimulated the HEPE formation. Among the metabolites of AA investigated, prostaglandin (PG)G2 and 12-hydroperoxy-5,8,10,14-eicosatetraenoic acid had the stimulatory effect on both TXB2 and HEPE formations, whereas PGH2, PGD2, TXB2 and 12-hydroxy-5, 8,10,14-eicosatetraenoic acid were ineffective.
KW - Arachidonic Acid Effect
KW - Eicosapentaenoic Acid Metabolism
UR - https://www.scopus.com/pages/publications/0022378168
U2 - 10.1016/0049-3848(85)90266-X
DO - 10.1016/0049-3848(85)90266-X
M3 - 記事
C2 - 3001967
AN - SCOPUS:0022378168
SN - 0049-3848
VL - 40
SP - 307
EP - 317
JO - Thrombosis Research
JF - Thrombosis Research
IS - 3
ER -