Effects of 5-methyl substitution in 2′-O-methyloligo-(pyrimidine)nucleotides on triple-helix formation

Miho Shimizu; Tatsuhiko Koizumi; Hideo Inoue; Eiko Ohtsuka

doi:10.1016/S0960-894X(01)80675-2

Effects of 5-methyl substitution in 2′-O-methyloligo-(pyrimidine)nucleotides on triple-helix formation

Miho Shimizu
, Tatsuhiko Koizumi
, Hideo Inoue
, Eiko Ohtsuka

Hokkaido University

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

A 2′-O_methyloligo(pyrimidine)nucleotide containing a 5-methyl-2′-O-methyluridine (I) forms a more stable triple-helix with double-stranded DNA than the 5-unmodified parent probe, whereas 5-methyl-2′-O-methylcytidine (II) destabilizes the triplex. It is also shown that at low salt concentrations, the triplexes with 2′-O-methyl RNAs are more stable than the corresponding DNA-triplex.

Original language	English
Pages (from-to)	1029-1032
Number of pages	4
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	4
Issue number	8
DOIs	https://doi.org/10.1016/S0960-894X(01)80675-2
State	Published - 21 Apr 1994
Externally published	Yes

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title = "Effects of 5-methyl substitution in 2′-O-methyloligo-(pyrimidine)nucleotides on triple-helix formation",

abstract = "A 2′-O\_methyloligo(pyrimidine)nucleotide containing a 5-methyl-2′-O-methyluridine (I) forms a more stable triple-helix with double-stranded DNA than the 5-unmodified parent probe, whereas 5-methyl-2′-O-methylcytidine (II) destabilizes the triplex. It is also shown that at low salt concentrations, the triplexes with 2′-O-methyl RNAs are more stable than the corresponding DNA-triplex.",

author = "Miho Shimizu and Tatsuhiko Koizumi and Hideo Inoue and Eiko Ohtsuka",

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T1 - Effects of 5-methyl substitution in 2′-O-methyloligo-(pyrimidine)nucleotides on triple-helix formation

AU - Shimizu, Miho

AU - Koizumi, Tatsuhiko

AU - Inoue, Hideo

AU - Ohtsuka, Eiko

PY - 1994/4/21

Y1 - 1994/4/21

N2 - A 2′-O_methyloligo(pyrimidine)nucleotide containing a 5-methyl-2′-O-methyluridine (I) forms a more stable triple-helix with double-stranded DNA than the 5-unmodified parent probe, whereas 5-methyl-2′-O-methylcytidine (II) destabilizes the triplex. It is also shown that at low salt concentrations, the triplexes with 2′-O-methyl RNAs are more stable than the corresponding DNA-triplex.

AB - A 2′-O_methyloligo(pyrimidine)nucleotide containing a 5-methyl-2′-O-methyluridine (I) forms a more stable triple-helix with double-stranded DNA than the 5-unmodified parent probe, whereas 5-methyl-2′-O-methylcytidine (II) destabilizes the triplex. It is also shown that at low salt concentrations, the triplexes with 2′-O-methyl RNAs are more stable than the corresponding DNA-triplex.

UR - https://www.scopus.com/pages/publications/0028212059

U2 - 10.1016/S0960-894X(01)80675-2

DO - 10.1016/S0960-894X(01)80675-2

M3 - 記事

AN - SCOPUS:0028212059

SN - 0960-894X

VL - 4

SP - 1029

EP - 1032

JO - Bioorganic and Medicinal Chemistry Letters

JF - Bioorganic and Medicinal Chemistry Letters

IS - 8

ER -

Effects of 5-methyl substitution in 2′-O-methyloligo-(pyrimidine)nucleotides on triple-helix formation

Abstract

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