DNA topoisomerase 2 gene polymorphism in dermatophytes

Forough Shamsizadeh; Ivan M. Pchelin; Koichi Makimura; Mohamed Mahdi Alshahni; Kazuo Satoh; Farzad Katiraee; Bahram Ahmadi; Ali Rezaei-Matehhkolaei

doi:10.1111/myc.13086

DNA topoisomerase 2 gene polymorphism in dermatophytes

Forough Shamsizadeh, Ivan M. Pchelin, Koichi Makimura, Mohamed Mahdi Alshahni, Kazuo Satoh, Farzad Katiraee, Bahram Ahmadi, Ali Rezaei-Matehhkolaei

General Medical Education and Research Center

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Background: Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives: Our goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes. Methods: We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results: The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2-ITS tree, one of its two clades contained solely Iranian isolates. Conclusions: Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.

Original language	English
Pages (from-to)	694-703
Number of pages	10
Journal	Mycoses
Volume	63
Issue number	7
DOIs	https://doi.org/10.1111/myc.13086
State	Published - 1 Jul 2020

Keywords

DNA topoisomerase 2
Nannizzia
Trichophyton
dermatophytes
species boundaries

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10.1111/myc.13086

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title = "DNA topoisomerase 2 gene polymorphism in dermatophytes",

abstract = "Background: Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives: Our goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes. Methods: We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results: The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2-ITS tree, one of its two clades contained solely Iranian isolates. Conclusions: Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.",

keywords = "DNA topoisomerase 2, Nannizzia, Trichophyton, dermatophytes, species boundaries",

author = "Forough Shamsizadeh and Pchelin, \{Ivan M.\} and Koichi Makimura and Alshahni, \{Mohamed Mahdi\} and Kazuo Satoh and Farzad Katiraee and Bahram Ahmadi and Ali Rezaei-Matehhkolaei",

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year = "2020",

month = jul,

day = "1",

doi = "10.1111/myc.13086",

language = "英語",

volume = "63",

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T1 - DNA topoisomerase 2 gene polymorphism in dermatophytes

AU - Shamsizadeh, Forough

AU - Pchelin, Ivan M.

AU - Makimura, Koichi

AU - Alshahni, Mohamed Mahdi

AU - Satoh, Kazuo

AU - Katiraee, Farzad

AU - Ahmadi, Bahram

AU - Rezaei-Matehhkolaei, Ali

PY - 2020/7/1

Y1 - 2020/7/1

N2 - Background: Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives: Our goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes. Methods: We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results: The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2-ITS tree, one of its two clades contained solely Iranian isolates. Conclusions: Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.

AB - Background: Dermatophytes are a group of keratinophilic fungi of medical importance. Despite a relatively long history of molecular taxonomic studies, there is still a need for information on genetic polymorphism in wider variety of genomic loci. Objectives: Our goal was to study partial DNA topoisomerase 2 gene (TOP2) polymorphism in dermatophytes. Methods: We performed DNA sequencing of TOP2 in 26 dermatophyte species along with ribosomal internal transcribed spacer (ITS) sequencing. Results: The number of polymorphic sites in TOP2 data set was similar to that one in ITS data set. Nannizzia species formed paraphyletic group in TOP2 tree. Trichophyton simii was paraphyletic in concatenated TOP2-ITS tree, one of its two clades contained solely Iranian isolates. Conclusions: Our results revealed several unresolved problems in the taxonomy of dermatophytes, including probable polyphyly of the genus Nannizzia and the species T simii.

KW - DNA topoisomerase 2

KW - Nannizzia

KW - Trichophyton

KW - dermatophytes

KW - species boundaries

UR - https://www.scopus.com/pages/publications/85084496816

U2 - 10.1111/myc.13086

DO - 10.1111/myc.13086

M3 - 記事

C2 - 32277529

AN - SCOPUS:85084496816

SN - 0933-7407

VL - 63

SP - 694

EP - 703

JO - Mycoses

JF - Mycoses

IS - 7

ER -

DNA topoisomerase 2 gene polymorphism in dermatophytes

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Keywords

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