Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate

Ken Ichi Mawatari; Motomasa Atsumi; Fumiya Nakamura; Makoto Yasuda; Tomoko Fukuuchi; Noriko Yamaoka; Kiyoko Kaneko; Kazuya Nakagomi; Naoto Oku

doi:10.1177/1178646919852120

Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate

Ken Ichi Mawatari
, Motomasa Atsumi
, Fumiya Nakamura
, Makoto Yasuda
, Tomoko Fukuuchi
, Noriko Yamaoka
, Kiyoko Kaneko
, Kazuya Nakagomi
, Naoto Oku

Faculty of Pharmaceutical Sciences

Teikyo University

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A system was developed for determining dipicolinic acid in “natto” using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).

Original language	English
Journal	International Journal of Tryptophan Research
Volume	12
DOIs	https://doi.org/10.1177/1178646919852120
State	Published - 1 Jun 2019

Keywords

dipicolinic acid
fluorescence detection
high performance liquid chromatography
UV irradiation
zinc acetate

Access to Document

10.1177/1178646919852120

Cite this

@article{385932b151684cd1975f4e420b6ca6ab,

title = "Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate",

abstract = "A system was developed for determining dipicolinic acid in “natto” using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).",

keywords = "dipicolinic acid, fluorescence detection, high performance liquid chromatography, UV irradiation, zinc acetate",

author = "Mawatari, \{Ken Ichi\} and Motomasa Atsumi and Fumiya Nakamura and Makoto Yasuda and Tomoko Fukuuchi and Noriko Yamaoka and Kiyoko Kaneko and Kazuya Nakagomi and Naoto Oku",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2019.",

year = "2019",

month = jun,

day = "1",

doi = "10.1177/1178646919852120",

language = "英語",

volume = "12",

journal = "International Journal of Tryptophan Research",

issn = "1178-6469",

publisher = "Libertas Academica Ltd.",

}

TY - JOUR

T1 - Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate

AU - Mawatari, Ken Ichi

AU - Atsumi, Motomasa

AU - Nakamura, Fumiya

AU - Yasuda, Makoto

AU - Fukuuchi, Tomoko

AU - Yamaoka, Noriko

AU - Kaneko, Kiyoko

AU - Nakagomi, Kazuya

AU - Oku, Naoto

PY - 2019/6/1

Y1 - 2019/6/1

N2 - A system was developed for determining dipicolinic acid in “natto” using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).

AB - A system was developed for determining dipicolinic acid in “natto” using liquid chromatography with fluorometric detection. The compound was separated by reversed-phase chromatography using a mobile phase of 0.1 mol/L disodium hydrogen phosphate, 0.05 mol/L citric acid buffer (adjusted to pH 3.0) containing 3.0 mmol/L zinc acetate and 35 mmol/L perchloric acid. The compound in the column effluent was irradiated with ultraviolet light to produce fluorescence. This fluorescence was monitored at an excitation at 336 nm and an emission at 448 nm. The calibration curve for dipicolinic acid was observed to be linear in a range of 0.2 to 112 ng. The dipicolinic acid content of natto was 7.24 ± 0.54 mg/100 g (wet weight, mean ± standard deviation [SD], n = 6).

KW - dipicolinic acid

KW - fluorescence detection

KW - high performance liquid chromatography

KW - UV irradiation

KW - zinc acetate

UR - https://www.scopus.com/pages/publications/85071327763

U2 - 10.1177/1178646919852120

DO - 10.1177/1178646919852120

M3 - 記事

AN - SCOPUS:85071327763

SN - 1178-6469

VL - 12

JO - International Journal of Tryptophan Research

JF - International Journal of Tryptophan Research

ER -

Determination of Dipicolinic Acid in “Natto” by High-Performance Liquid Chromatography Coupled With Postcolumn Photoirradiation With Zinc Acetate

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this