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Detection of prothrombin and osteopontin in a renal stone found in a hyperuricemic patient using 2D-PAGE and LC-MS analysis

  • K. Kaneko
  • , T. Yamanobe
  • , K. Nakagomi
  • , K. Mawatari
  • , M. Onoda
  • , S. Fujimori
  • Teikyo University

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The liquid chromatography-mass spectrometry (LC-MS) following on from the two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) technique was applied for the analysis of proteins in a renal stone found in a hyperuricemic patient. This technique was sensitive enough to detect small quantities of proteins even in a renal stone.

Original languageEnglish
Pages (from-to)1127-1129
Number of pages3
JournalNucleosides, Nucleotides and Nucleic Acids
Volume23
Issue number8-9
DOIs
StatePublished - 2004

Keywords

  • 2D-PAGE and LC-MS
  • Analysis of protein
  • Osteopontin
  • Prothrombin
  • Renal stone

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