Damage measurement on T-cells and analysis of parameter dependency under ultrasound exposure

Because ultrasound irradiation causes damage on cells, we analyzed the damage on cells under ultrasound exposure by varying its parameters. We examined the suspension of T-cells of mice, in a well plate on the surface of the degassed water at a water temperature of 37 ℃. From the bottom of a water tank, ultrasound irradiation was performed by targeting a suspension of cells. After irradiation, fluorescent reagents were used to calculate the cell viability. As a result, the survival rate was the lowest at a frequency of 3 MHz, when a continuous wave (100% of duty ratio) was applied with a sound pressure of 400 kPa-pp. On the other hand, when only the duty ratio is less than 20% without changing other parameters, the survival rate raised 100%. From the results, cell damage complexly related to various ultrasound parameters.