Culture of mouse cartilage cells on DNA films

Fuyuko Tanabe; Yukari Suzuki; Amane Watanuki; Masatoshi Kuroda; Youichi Takahashi; Naoshi Ogata; Naoya Ogata

doi:10.1295/koron.69.33

Culture of mouse cartilage cells on DNA films

Fuyuko Tanabe
, Yukari Suzuki
, Amane Watanuki
, Masatoshi Kuroda
, Youichi Takahashi
, Naoshi Ogata
, Naoya Ogata

Ogata Research Laboratory, Ltd
Dainippon Printing Company
The University of Tokyo

Research output: Contribution to journal › Article › peer-review

Abstract

Pure deoxyribonucleic acid (DMA) whicn was obtained from Salmon roe was dissolved in water, followed by evaporating water to obtain clear and mechanically strong films. Since DNA is proved to be biocompatible as biomaterials, water-insoluble DNA films were prepared to apply to culture plates for mouse cartilage cells. DNA films were irradiated by UV lamp to obtain water-insoluble cross-linked DNA films which were used for cell culture. It was found that the UV cured DNA films were suitable for mouse cartilage cell culture and cells can grow up to higher cell density on UV cured DNA films than normal cell culture dishes.

Original language	English
Pages (from-to)	33-38
Number of pages	6
Journal	Kobunshi Ronbunshu
Volume	69
Issue number	1
DOIs	https://doi.org/10.1295/koron.69.33
State	Published - Jan 2012
Externally published	Yes

Keywords

Cartilage cell
Cell culture
DNA
DNA-UV cured film

Access to Document

10.1295/koron.69.33

Cite this

@article{e11a4ac0b9bf43a996dbadcffaf3593e,

title = "Culture of mouse cartilage cells on DNA films",

abstract = "Pure deoxyribonucleic acid (DMA) whicn was obtained from Salmon roe was dissolved in water, followed by evaporating water to obtain clear and mechanically strong films. Since DNA is proved to be biocompatible as biomaterials, water-insoluble DNA films were prepared to apply to culture plates for mouse cartilage cells. DNA films were irradiated by UV lamp to obtain water-insoluble cross-linked DNA films which were used for cell culture. It was found that the UV cured DNA films were suitable for mouse cartilage cell culture and cells can grow up to higher cell density on UV cured DNA films than normal cell culture dishes.",

keywords = "Cartilage cell, Cell culture, DNA, DNA-UV cured film",

author = "Fuyuko Tanabe and Yukari Suzuki and Amane Watanuki and Masatoshi Kuroda and Youichi Takahashi and Naoshi Ogata and Naoya Ogata",

year = "2012",

month = jan,

doi = "10.1295/koron.69.33",

language = "英語",

volume = "69",

pages = "33--38",

journal = "Kobunshi Ronbunshu",

issn = "0386-2186",

publisher = "Society of Polymer Science",

number = "1",

}

TY - JOUR

T1 - Culture of mouse cartilage cells on DNA films

AU - Tanabe, Fuyuko

AU - Suzuki, Yukari

AU - Watanuki, Amane

AU - Kuroda, Masatoshi

AU - Takahashi, Youichi

AU - Ogata, Naoshi

AU - Ogata, Naoya

PY - 2012/1

Y1 - 2012/1

N2 - Pure deoxyribonucleic acid (DMA) whicn was obtained from Salmon roe was dissolved in water, followed by evaporating water to obtain clear and mechanically strong films. Since DNA is proved to be biocompatible as biomaterials, water-insoluble DNA films were prepared to apply to culture plates for mouse cartilage cells. DNA films were irradiated by UV lamp to obtain water-insoluble cross-linked DNA films which were used for cell culture. It was found that the UV cured DNA films were suitable for mouse cartilage cell culture and cells can grow up to higher cell density on UV cured DNA films than normal cell culture dishes.

AB - Pure deoxyribonucleic acid (DMA) whicn was obtained from Salmon roe was dissolved in water, followed by evaporating water to obtain clear and mechanically strong films. Since DNA is proved to be biocompatible as biomaterials, water-insoluble DNA films were prepared to apply to culture plates for mouse cartilage cells. DNA films were irradiated by UV lamp to obtain water-insoluble cross-linked DNA films which were used for cell culture. It was found that the UV cured DNA films were suitable for mouse cartilage cell culture and cells can grow up to higher cell density on UV cured DNA films than normal cell culture dishes.

KW - Cartilage cell

KW - Cell culture

KW - DNA

KW - DNA-UV cured film

UR - https://www.scopus.com/pages/publications/84857618566

U2 - 10.1295/koron.69.33

DO - 10.1295/koron.69.33

M3 - 記事

AN - SCOPUS:84857618566

SN - 0386-2186

VL - 69

SP - 33

EP - 38

JO - Kobunshi Ronbunshu

JF - Kobunshi Ronbunshu

IS - 1

ER -

Culture of mouse cartilage cells on DNA films

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this