Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

Yoshio Kusakabe; Satoshi Ohno; Nobutada Tanaka; Masashi Nakamura; Masaru Tsunoda; Tomohisa Moriguchi; Norio Asai; Mitsuo Sekine; Takashi Yokogawa; Kazuya Nishikawa; Kazuo T. Nakamura

doi:10.2174/092986606775974465

Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

Yoshio Kusakabe, Satoshi Ohno, Nobutada Tanaka, Masashi Nakamura, Masaru Tsunoda, Tomohisa Moriguchi, Norio Asai, Mitsuo Sekine, Takashi Yokogawa, Kazuya Nishikawa, Kazuo T. Nakamura

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNA^Tyr. The crystals belong to a tetragonal space group P4₁2₁2 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNA^Tyr (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Å resolution.

Original language	English
Pages (from-to)	417-419
Number of pages	3
Journal	Protein and Peptide Letters
Volume	13
Issue number	4
DOIs	https://doi.org/10.2174/092986606775974465
State	Published - Apr 2006
Externally published	Yes

Keywords

Aminoacyl-tRNA synthetase
Crystallization
tRNA
TyrRS

Access to Document

10.2174/092986606775974465

Cite this

Kusakabe, Y., Ohno, S., Tanaka, N., Nakamura, M., Tsunoda, M., Moriguchi, T., Asai, N., Sekine, M., Yokogawa, T., Nishikawa, K., & Nakamura, K. T. (2006). Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA. Protein and Peptide Letters, 13(4), 417-419. https://doi.org/10.2174/092986606775974465

@article{323626c93e9741f7ae8e97db03358911,

title = "Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA",

abstract = "Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 {\AA}, and c = 330.3 {\AA}. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 {\AA} resolution.",

keywords = "Aminoacyl-tRNA synthetase, Crystallization, tRNA, TyrRS",

author = "Yoshio Kusakabe and Satoshi Ohno and Nobutada Tanaka and Masashi Nakamura and Masaru Tsunoda and Tomohisa Moriguchi and Norio Asai and Mitsuo Sekine and Takashi Yokogawa and Kazuya Nishikawa and Nakamura, {Kazuo T.}",

year = "2006",

month = apr,

doi = "10.2174/092986606775974465",

language = "英語",

volume = "13",

pages = "417--419",

journal = "Protein and Peptide Letters",

issn = "0929-8665",

publisher = "Bentham Science Publishers",

number = "4",

}

Kusakabe, Y, Ohno, S, Tanaka, N, Nakamura, M, Tsunoda, M, Moriguchi, T, Asai, N, Sekine, M, Yokogawa, T, Nishikawa, K & Nakamura, KT 2006, 'Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA', Protein and Peptide Letters, vol. 13, no. 4, pp. 417-419. https://doi.org/10.2174/092986606775974465

TY - JOUR

T1 - Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

AU - Kusakabe, Yoshio

AU - Ohno, Satoshi

AU - Tanaka, Nobutada

AU - Nakamura, Masashi

AU - Tsunoda, Masaru

AU - Moriguchi, Tomohisa

AU - Asai, Norio

AU - Sekine, Mitsuo

AU - Yokogawa, Takashi

AU - Nishikawa, Kazuya

AU - Nakamura, Kazuo T.

PY - 2006/4

Y1 - 2006/4

N2 - Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Å resolution.

AB - Yeast tyrosyl-tRNA synthetase (yTyrRS) has been crystallized by the vapor diffusion method in the presence of its cognate tRNATyr. The crystals belong to a tetragonal space group P41212 with cell dimensions of a = b = 63.85 Å, and c = 330.3 Å. The asymmetric unit contains one molecule each of yTyrRS and tRNATyr (one-half of a 2:2 complex). X-ray diffraction data have been collected up to 2.5 Å resolution.

KW - Aminoacyl-tRNA synthetase

KW - Crystallization

KW - tRNA

KW - TyrRS

UR - http://www.scopus.com/inward/record.url?scp=33646433470&partnerID=8YFLogxK

U2 - 10.2174/092986606775974465

DO - 10.2174/092986606775974465

M3 - 記事

C2 - 16712521

AN - SCOPUS:33646433470

SN - 0929-8665

VL - 13

SP - 417

EP - 419

JO - Protein and Peptide Letters

JF - Protein and Peptide Letters

IS - 4

ER -

Crystallization and preliminary X-ray crystallographic analysis of yeast tyrosyl-tRNA synthetase complexed with its cognate tRNA

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this