Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

Dimitri T. Azar; Fabio H. Casanova; Tatsuya Mimura; Sandeep Jain; Zhongjun Zhou; Kyu Yeon Han; Jin Hong Chang

doi:10.1097/ICO.0b013e3181b1165d

Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

Dimitri T. Azar, Fabio H. Casanova, Tatsuya Mimura, Sandeep Jain, Zhongjun Zhou, Kyu Yeon Han, Jin Hong Chang

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

Original language	English
Pages (from-to)	321-330
Number of pages	10
Journal	Cornea
Volume	29
Issue number	3
DOIs	https://doi.org/10.1097/ICO.0b013e3181b1165d
State	Published - Mar 2010
Externally published	Yes

Keywords

Cornea
Epithelium
MT1-MMP
TIMP

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1097/ICO.0b013e3181b1165d

Cite this

@article{b932270777f3445fba762bafd06b0bce,

title = "Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration",

abstract = "Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.",

keywords = "Cornea, Epithelium, MT1-MMP, TIMP",

author = "Azar, \{Dimitri T.\} and Casanova, \{Fabio H.\} and Tatsuya Mimura and Sandeep Jain and Zhongjun Zhou and Han, \{Kyu Yeon\} and Chang, \{Jin Hong\}",

year = "2010",

month = mar,

doi = "10.1097/ICO.0b013e3181b1165d",

language = "英語",

volume = "29",

pages = "321--330",

journal = "Cornea",

issn = "0277-3740",

publisher = "Lippincott Williams and Wilkins",

number = "3",

}

TY - JOUR

T1 - Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

AU - Azar, Dimitri T.

AU - Casanova, Fabio H.

AU - Mimura, Tatsuya

AU - Jain, Sandeep

AU - Zhou, Zhongjun

AU - Han, Kyu Yeon

AU - Chang, Jin Hong

PY - 2010/3

Y1 - 2010/3

N2 - Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

AB - Purpose: To determine the effects of corneal epithelial membrane-type 1 matrix metalloproteinase (MT1-MMP) on vascular endothelial migration and proliferation. Methods: We generated immortalized wild-type, MT1-MMP knockout and MT1-MMP knock-in corneal epithelial cells. Calf pulmonary arterial endothelial (CPAE) cell proliferation and Boyden chamber migration were assayed. Results: Conditioned media from MT1-MMP epithelial knockout cells significantly increased CPAE proliferation 5-bromo-2'-deoxy-uridine (BrdU) incorporation, and CPAE migration as compared with wild-type epithelial cells. Conditioned media from knock-in cells reversed the increase in CPAE proliferation, BrdU incorporation and CPAE migration. Knock-in cells transfected with mutant MT1-MMP (E240A) did not abrogate the reversal effect. Conclusions: Corneal epithelial MT1-MMP is antiangiogenic. This antiangiogenic activity does not require the catalytic domain.

KW - Cornea

KW - Epithelium

KW - MT1-MMP

KW - TIMP

UR - https://www.scopus.com/pages/publications/77649164902

U2 - 10.1097/ICO.0b013e3181b1165d

DO - 10.1097/ICO.0b013e3181b1165d

M3 - 記事

C2 - 20118785

AN - SCOPUS:77649164902

SN - 0277-3740

VL - 29

SP - 321

EP - 330

JO - Cornea

JF - Cornea

IS - 3

ER -

Corneal epithelial MT1-MMP inhibits vascular endothelial cell proliferation and migration

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this