Comparative effects of basophil-directed growth factors

Chitose Yoshimura-Uchiyama; Masao Yamaguchi; Hiroyuki Nagase; Takao Fujisawa; Chisei Ra; Kouji Matsushima; Tsutomu Iwata; Takashi Igarashi; Kazuhiko Yamamoto; Koichi Hirai

doi:10.1016/S0006-291X(03)00153-0

Comparative effects of basophil-directed growth factors

Chitose Yoshimura-Uchiyama
, Masao Yamaguchi
, Hiroyuki Nagase
, Takao Fujisawa
, Chisei Ra
, Kouji Matsushima
, Tsutomu Iwata
, Takashi Igarashi
, Kazuhiko Yamamoto
, Koichi Hirai

Research output: Contribution to journal › Article › peer-review

33 Scopus citations

Abstract

IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Rα>IL-5Rα>GM-CSFRα in basophils and IL-5Rα≥GM-CSFRα>IL-3Rα in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Rα and similar levels of IL-5Rα and GM-CSFRα. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF≥IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.

Original language	English
Pages (from-to)	201-206
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	302
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(03)00153-0
State	Published - 10 Jan 2003
Externally published	Yes

Keywords

Apoptosis
Basophils
CD11b
CD69
Degranulation
Eosinophils
GM-CSF
IL-3
IL-5
Receptor

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10.1016/S0006-291X(03)00153-0

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title = "Comparative effects of basophil-directed growth factors",

abstract = "IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Rα>IL-5Rα>GM-CSFRα in basophils and IL-5Rα≥GM-CSFRα>IL-3Rα in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Rα and similar levels of IL-5Rα and GM-CSFRα. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF≥IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.",

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author = "Chitose Yoshimura-Uchiyama and Masao Yamaguchi and Hiroyuki Nagase and Takao Fujisawa and Chisei Ra and Kouji Matsushima and Tsutomu Iwata and Takashi Igarashi and Kazuhiko Yamamoto and Koichi Hirai",

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AU - Yoshimura-Uchiyama, Chitose

AU - Yamaguchi, Masao

AU - Nagase, Hiroyuki

AU - Fujisawa, Takao

AU - Ra, Chisei

AU - Matsushima, Kouji

AU - Iwata, Tsutomu

AU - Igarashi, Takashi

AU - Yamamoto, Kazuhiko

AU - Hirai, Koichi

PY - 2003/1/10

Y1 - 2003/1/10

N2 - IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Rα>IL-5Rα>GM-CSFRα in basophils and IL-5Rα≥GM-CSFRα>IL-3Rα in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Rα and similar levels of IL-5Rα and GM-CSFRα. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF≥IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.

AB - IL-3, IL-5, and GM-CSF exert various overlapping functions in basophils. We investigated the receptor expression profiles and concentration-dependent effects of IL-3, IL-5, and GM-CSF on several basophil functions in comparison with their effects on eosinophils. The order of the receptor expression levels was IL-3Rα>IL-5Rα>GM-CSFRα in basophils and IL-5Rα≥GM-CSFRα>IL-3Rα in eosinophils. Compared with eosinophils, basophils expressed a much higher level of IL-3Rα and similar levels of IL-5Rα and GM-CSFRα. The order of potency was IL-3>IL-5=GM-CSF for degranulation, survival, and CD11b expression in basophils, and IL-5=GM-CSF≥IL-3 for survival and CD11b expression in eosinophils. However, IL-3 induced CD69 expression preferentially in basophils. Our results indicate that IL-3 is the most potent activator of human basophils, and that the rank order of potency of hemopoietic growth factors virtually corresponded to their receptor expression levels in both cell types.

KW - Apoptosis

KW - Basophils

KW - CD11b

KW - CD69

KW - Degranulation

KW - Eosinophils

KW - GM-CSF

KW - IL-3

KW - IL-5

KW - Receptor

UR - https://www.scopus.com/pages/publications/0037428287

U2 - 10.1016/S0006-291X(03)00153-0

DO - 10.1016/S0006-291X(03)00153-0

M3 - 記事

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AN - SCOPUS:0037428287

SN - 0006-291X

VL - 302

SP - 201

EP - 206

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 2

ER -

Comparative effects of basophil-directed growth factors

Abstract

Keywords

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