Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome

Akira Matsuno; Johbu Itoh; Akiko Mizutani; Susumu Takekoshi; R. Yoshiyuki Osamura; Hiroko Okinaga; Fuyuaki Ide; Satoru Miyawaki; Takeshi Uno; Shuichiro Asano; Junichi Tanaka; Hiroshi Nakaguchi; Mitsuyoshi Sasaki; Mineko Murakami

doi:10.2478/v10042-008-0069-5

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome

Akira Matsuno, Johbu Itoh, Akiko Mizutani, Susumu Takekoshi, R. Yoshiyuki Osamura, Hiroko Okinaga, Fuyuaki Ide, Satoru Miyawaki, Takeshi Uno, Shuichiro Asano, Junichi Tanaka, Hiroshi Nakaguchi, Mitsuyoshi Sasaki, Mineko Murakami

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

Original language	English
Pages (from-to)	419-421
Number of pages	3
Journal	Folia Histochemica et Cytobiologica
Volume	46
Issue number	4
DOIs	https://doi.org/10.2478/v10042-008-0069-5
State	Published - 2008
Externally published	Yes

Keywords

ECFP
EYFP
GH
Porosome
rab3B

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10.2478/v10042-008-0069-5

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Matsuno, A., Itoh, J., Mizutani, A., Takekoshi, S., Osamura, R. Y., Okinaga, H., Ide, F., Miyawaki, S., Uno, T., Asano, S., Tanaka, J., Nakaguchi, H., Sasaki, M., & Murakami, M. (2008). Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome. Folia Histochemica et Cytobiologica, 46(4), 419-421. https://doi.org/10.2478/v10042-008-0069-5

@article{f155737af7204232a535f28a6e890ddf,

title = "Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome",

abstract = "Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.",

keywords = "ECFP, EYFP, GH, Porosome, rab3B",

author = "Akira Matsuno and Johbu Itoh and Akiko Mizutani and Susumu Takekoshi and Osamura, \{R. Yoshiyuki\} and Hiroko Okinaga and Fuyuaki Ide and Satoru Miyawaki and Takeshi Uno and Shuichiro Asano and Junichi Tanaka and Hiroshi Nakaguchi and Mitsuyoshi Sasaki and Mineko Murakami",

year = "2008",

doi = "10.2478/v10042-008-0069-5",

language = "英語",

volume = "46",

pages = "419--421",

journal = "Folia Histochemica et Cytobiologica",

issn = "0239-8508",

publisher = "Via Medica",

number = "4",

}

Matsuno, A, Itoh, J, Mizutani, A, Takekoshi, S, Osamura, RY, Okinaga, H, Ide, F, Miyawaki, S, Uno, T, Asano, S, Tanaka, J, Nakaguchi, H, Sasaki, M & Murakami, M 2008, 'Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome', Folia Histochemica et Cytobiologica, vol. 46, no. 4, pp. 419-421. https://doi.org/10.2478/v10042-008-0069-5

TY - JOUR

T1 - Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell

T2 - A role of rab3B in secretion of GH through porosome

AU - Matsuno, Akira

AU - Itoh, Johbu

AU - Mizutani, Akiko

AU - Takekoshi, Susumu

AU - Osamura, R. Yoshiyuki

AU - Okinaga, Hiroko

AU - Ide, Fuyuaki

AU - Miyawaki, Satoru

AU - Uno, Takeshi

AU - Asano, Shuichiro

AU - Tanaka, Junichi

AU - Nakaguchi, Hiroshi

AU - Sasaki, Mitsuyoshi

AU - Murakami, Mineko

PY - 2008

Y1 - 2008

N2 - Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

AB - Recently, in order to elucidate the role of rab3B in porosome, we have observed the incorporation of rab3B in the secretion of GH through porosome under confocal laser scanning microscopy (CLSM). Transfected cells with GH-EYFP fusion protein and rab3B-ECFP fusion protein were observed under CLSM, which showed the colocalization of EYFP-GH and ECFP-rab3B in the budding configuration of secretory process. These structural and functional images of rab3B imply the incorporation of rab3B in the secretion of GH through porosome.

KW - ECFP

KW - EYFP

KW - GH

KW - Porosome

KW - rab3B

UR - https://www.scopus.com/pages/publications/60549104224

U2 - 10.2478/v10042-008-0069-5

DO - 10.2478/v10042-008-0069-5

M3 - 記事

C2 - 19141391

AN - SCOPUS:60549104224

SN - 0239-8508

VL - 46

SP - 419

EP - 421

JO - Folia Histochemica et Cytobiologica

JF - Folia Histochemica et Cytobiologica

IS - 4

ER -

Co-transfection of EYFP-GH and ECFP-rab3B in an experimental pituitary GH3 cell: A role of rab3B in secretion of GH through porosome

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