@inproceedings{1d51fe8b86df479597352bc599221672,
title = "Characterisation of gene delivery using liposomal Bubbles and ultrasound",
abstract = "The combination of nano/microbubbles and ultrasound is a novel technique for a nonviral gene deliver. We have previously developed novel ultrasound sensitive liposomes (Bubble liposomes) which contain the ultrasound imaging gas perfluoropropane. In this study, Bubble liposomes were compared with cationic lipid (CL)-DNA complexes as potential gene delivery carriers into tumors in vivo. The delivery of genes by bubble liposomes depended on the intensity of the applied ultrasound. The transfection efficiency plateaued at 0.7 W/cm 2 ultrasound intensity. Bubble liposomes efficiently transferred genes into cultured cells even when the cells were exposed to ultrasound for only 1 s. In addition, bubble liposomes were able to introduce the luciferase gene more effectively than CL-DNA complexes into mouse ascites tumor cells. We conclude that the combination of Bubble liposomes and ultrasound is a good method for gene transfer in vivo.",
keywords = "Gene delivery, Liposome, Nanobubble, Non-viral vector, Sonoporation",
author = "Risa Koshima and Ryo Suzuki and Yusuke Oda and Keiichi Hirata and Tetsuya Nomura and Yoichi Negishi and Naoki Utoguchi and Nobuki Kudo and Kazuo Maruyama",
year = "2011",
doi = "10.1063/1.3607928",
language = "英語",
isbn = "9780735409170",
series = "AIP Conference Proceedings",
pages = "330--334",
booktitle = "10th International Symposium on Therapeutic Ultrasound, ISTU 2010",
note = "10th International Symposium on Therapeutic Ultrasound, ISTU 2010 ; Conference date: 09-06-2010 Through 12-06-2010",
}