Analysis of PPAR alpha function in human kidney cell line using siRNA.

Keisuke Tachibana; Naohiko Anzai; Chihiro Ueda; Tatsuya Katayama; Takayoshi Kirino; Rika Takahashi; Daisuke Yamasaki; Kenji Ishimoto; Toshiya Tanaka; Takao Hamakubo; Yukihiko Ueda; Hiroyuki Arai; Juro Sakai; Tatsuhiko Kodama; Takefumi Doi

doi:10.1093/nass/nrl128

Analysis of PPAR alpha function in human kidney cell line using siRNA.

Keisuke Tachibana
, Naohiko Anzai
, Chihiro Ueda
, Tatsuya Katayama
, Takayoshi Kirino
, Rika Takahashi
, Daisuke Yamasaki
, Kenji Ishimoto
, Toshiya Tanaka
, Takao Hamakubo
, Yukihiko Ueda
, Hiroyuki Arai
, Juro Sakai
, Tatsuhiko Kodama
, Takefumi Doi

The University of Osaka

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.

Original language	English
Pages (from-to)	257-258
Number of pages	2
Journal	Nucleic acids symposium series
Issue number	50
DOIs	https://doi.org/10.1093/nass/nrl128
State	Published - 2006
Externally published	Yes

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10.1093/nass/nrl128

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title = "Analysis of PPAR alpha function in human kidney cell line using siRNA.",

abstract = "The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.",

author = "Keisuke Tachibana and Naohiko Anzai and Chihiro Ueda and Tatsuya Katayama and Takayoshi Kirino and Rika Takahashi and Daisuke Yamasaki and Kenji Ishimoto and Toshiya Tanaka and Takao Hamakubo and Yukihiko Ueda and Hiroyuki Arai and Juro Sakai and Tatsuhiko Kodama and Takefumi Doi",

year = "2006",

doi = "10.1093/nass/nrl128",

language = "英語",

pages = "257--258",

journal = "Nucleic acids symposium series",

issn = "0261-3166",

publisher = "Oxford University Press",

number = "50",

}

TY - JOUR

T1 - Analysis of PPAR alpha function in human kidney cell line using siRNA.

AU - Tachibana, Keisuke

AU - Anzai, Naohiko

AU - Ueda, Chihiro

AU - Katayama, Tatsuya

AU - Kirino, Takayoshi

AU - Takahashi, Rika

AU - Yamasaki, Daisuke

AU - Ishimoto, Kenji

AU - Tanaka, Toshiya

AU - Hamakubo, Takao

AU - Ueda, Yukihiko

AU - Arai, Hiroyuki

AU - Sakai, Juro

AU - Kodama, Tatsuhiko

AU - Doi, Takefumi

PY - 2006

Y1 - 2006

N2 - The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.

AB - The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. PPARalpha is mainly expressed in the liver, kidney, heart and muscle. PPARalpha activates fatty acid catabolism, stimulates gluconeogenesis and ketone body synthesis and is involved in the control of lipoprotein assembly. Although PPARalpha is well characterized in the liver, its physiological function is unknown in the kidney. To investigate the intimate function of PPARalpha in the kidney, we analyzed the target gene expression in human metastatic renal cell carcinoma cell line, Caki-1, using small interfering RNA (siRNA) against PPARalpha and real-time RT-PCR methods. We found that some selected genes (long-chain fatty-acid-CoA ligase (FACL1), carnitine palmitoyltransferase 1A (CPT1A), adipose differentiation-related protein (ADRP) and aquaporin 3 (AQP3)) were down-regulated by PPARalpha siRNA. These results suggest that PPARalpha regulates fatty acid metabolism and body water homeostasis in this cell line.

UR - https://www.scopus.com/pages/publications/36049041867

U2 - 10.1093/nass/nrl128

DO - 10.1093/nass/nrl128

M3 - 記事

C2 - 17150915

AN - SCOPUS:36049041867

SN - 0261-3166

SP - 257

EP - 258

JO - Nucleic acids symposium series

JF - Nucleic acids symposium series

IS - 50

ER -

Analysis of PPAR alpha function in human kidney cell line using siRNA.

Abstract

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